Department of Animal and Veterinary Science, University of Idaho, Moscow, Idaho 83844, USA.
Nanomedicine. 2010 Feb;6(1):84-92. doi: 10.1016/j.nano.2009.03.003. Epub 2009 May 15.
Proliferating cells representing two disease models (HeLa and Panc 10.05 cells) and a more physiologically relevant cell model (3T3-L1 cells) were used to study the acute toxicologic effects of silica nanowires (NWs). Cellular responses to NW effects were determined over a 4- to 20-hour exposure time-course. Proliferation, viability, metabolic activity, and toxicologic mechanism (apoptosis vs. necrosis) data showed the following: 3 x 10(4) NWs per cell inhibited cell proliferation. The effect was rapid in HeLa cells, but 3T3-L1 and Panc 10.05 cells appeared to be more tolerant to NWs, effects being significant only after 20 or 16 hours, respectively. Cells of all three cell lines showed a significant reduction in cellular metabolic activity after 20 hours of treatment with NWs. Assay of NW-invoked mechanism of cell death (caspase 3/7 activity) indicated that apoptosis was minimally induced. Small but significant effects of NWs were detected in 3T3-L1 and HeLa cells after 20-hour treatment. No NW-induction of caspase 3/7 activity was detected for Panc 10.05 cells. Proliferating cells provide a sensitive model to study treatment with silica NWs. Silica NWs appeared to be well tolerated by these three cell lines at the doses tested. When effects were detected, cell necrosis and not apoptosis was the main mechanism of silica NW-induced cell death.
In this study, three relevant cell culture models were used to study the acute toxicological effects of silica nanowires (NW). All cell lines cells showed a significant reduction in cellular metabolic activity after 20 h of treatment with NW. Overall, silica NW appeared to be well-tolerated by these cell lines at the tested doses. Cell necrosis as opposed to apoptosis was the main mechanism of silica NW-induced cell death.
使用两种疾病模型（HeLa 和 Panc 10.05 细胞）和一种更具生理相关性的细胞模型（3T3-L1 细胞）的增殖细胞来研究二氧化硅纳米线（NW）的急性毒性作用。在 4-20 小时的暴露时间过程中，确定了细胞对 NW 影响的反应。增殖、活力、代谢活性和毒性机制（凋亡与坏死）数据表明：每个细胞 3x10(4)NW 抑制细胞增殖。HeLa 细胞中的这种作用是快速的，但 3T3-L1 和 Panc 10.05 细胞似乎对 NW 更耐受，只有在 20 或 16 小时后才会产生明显的效果。三种细胞系的细胞在 20 小时的 NW 处理后，细胞代谢活性均显著降低。检测 NW 诱导的细胞死亡机制（半胱天冬酶 3/7 活性）表明，凋亡的诱导最小。在 20 小时的治疗后，在 3T3-L1 和 HeLa 细胞中检测到 NW 的微小但显著的作用。对于 Panc 10.05 细胞，未检测到 NW 诱导的半胱天冬酶 3/7 活性。增殖细胞提供了研究用二氧化硅 NW 治疗的敏感模型。在测试剂量下，这三种细胞系似乎都能很好地耐受二氧化硅 NW。当检测到效果时，细胞坏死而不是细胞凋亡是二氧化硅 NW 诱导细胞死亡的主要机制。
在这项研究中，使用三种相关的细胞培养模型来研究二氧化硅纳米线（NW）的急性毒性作用。所有细胞系在 NW 处理 20 小时后，细胞代谢活性均显著降低。总的来说，在测试剂量下，这些细胞系对二氧化硅 NW 表现出良好的耐受性。细胞坏死而非细胞凋亡是二氧化硅 NW 诱导细胞死亡的主要机制。
