College of Chemistry and Chemical Engineering, Anhui University, Hefei, Anhui 230039, PR China.
Biomaterials. 2010 Mar;31(7):1509-17. doi: 10.1016/j.biomaterials.2009.11.034. Epub 2009 Nov 28.
The toxicity of nanostructured materials and nanoparticles are very important considerations for many nanotechnology applications. Iron nanowires (NWs), as useful magnetic nanomaterials, may be good candidates for several biomedical applications. Here Fe NWs with an average diameter of about 50 nm were prepared by electrodeposition within the nanopores of anodic aluminum oxide (AAO) templates, and characterized by using scanning electron microcopy (SEM), transmission electron microscopy (TEM) and vibrating sample magnetometer (VSM). The cytotoxicity of the Fe NWs was studied at cell level. Specifically, the influence of concentration and size on the cytotoxicity of Fe NWs to HeLa cells was evaluated by MTT assay combined with direct morphology observations by TEM and phase contrast microscopy. The results clearly showed that the presence of Fe NWs had no significant effect on the cell proliferation and cell viability, even the HeLa cells exposed to Fe NWs at the high concentration of 10,000 per cell for 72 h still showed high cell viability of about 80%. From phase contrast microscopy, confocal laser scanning microscopy (CLSM) and TEM observations, it was found that Fe NWs were indeed internalized by HeLa cells. The cellular uptake process and results of Fe NWs demonstrated that as-prepared Fe NWs had not only a good biocompatibility but also a very low cytotoxicity.
纳米结构材料和纳米粒子的毒性是许多纳米技术应用中非常重要的考虑因素。铁纳米线（NWs）作为有用的磁性纳米材料，可能是几种生物医学应用的良好候选材料。这里通过在阳极氧化铝（AAO）模板的纳米孔内电沉积制备了平均直径约为 50nm 的 Fe NWs，并通过扫描电子显微镜（SEM）、透射电子显微镜（TEM）和振动样品磁强计（VSM）进行了表征。在细胞水平上研究了 Fe NWs 的细胞毒性。具体来说，通过 MTT 分析结合 TEM 和相差显微镜的直接形态观察，评估了浓度和尺寸对 HeLa 细胞中 Fe NWs 细胞毒性的影响。结果清楚地表明，Fe NWs 的存在对细胞增殖和细胞活力没有显著影响，即使 HeLa 细胞在 10,000 个/细胞的高浓度下暴露 72 小时，细胞活力仍约为 80%。从相差显微镜、共聚焦激光扫描显微镜（CLSM）和 TEM 观察中发现，Fe NWs 确实被 HeLa 细胞内化。细胞摄取过程和 Fe NWs 的结果表明，所制备的 Fe NWs 不仅具有良好的生物相容性，而且细胞毒性非常低。
