Lagonegro P, Rossi F, Galli C, Smerieri A, Alinovi R, Pinelli S, Rimoldi T, Attolini G, Macaluso G, Macaluso C, Saddow S E, Salviati G
IMEM-CNR Institute, Parco Area delle Scienze 37/A, 43124 Parma, Italy.
IMEM-CNR Institute, Parco Area delle Scienze 37/A, 43124 Parma, Italy; Department of Biomedical, Biotechnological, and Translational Sciences, Parma University, via Gramsci 14, 43126 Parma, Italy.
Mater Sci Eng C Mater Biol Appl. 2017 Apr 1;73:465-471. doi: 10.1016/j.msec.2016.12.096. Epub 2016 Dec 21.
Nanowires are promising biomaterials in multiple clinical applications. The goal of this study was to investigate the cytotoxicity of carbon-doped silica nanowires (SiOC NWs) on a fibroblastic cell line in vitro.
SiOC NWs were grown on Si substrates by CVD process. Murine L929 fibroblasts were cultured in complete DMEM and indirect and direct cytotoxicity tests were performed in agreement with ISO 19003-5, by quantitating cell viability at MTT and chemiluminescent assay. Cell cultures were investigated at Scanning Electron Microscope (SEM) and immunocytochemistry to observe their morphology and investigate cell-NWs interactions. Furthermore, hemocompatibility with Platelet-rich Plasma was assayed at SEM and by ELISA assay.
SiOxCy NWs proved biocompatible and did not impair cell proliferation at contact assays. L929 were able to attach on NWs and proliferate. Most interestingly, L929 reorganised the NW scaffold by displacing the nanostructure and creating tunnels within the NW network. NWs moreover did not impair platelet activation and behaved similarly to flat SiO.
Our data show that SiOxCy NWs did not release cytotoxic species and acted as a viable and adaptable scaffold for fibroblastic cells, thus representing a promising platform for implantable devices.
纳米线在多种临床应用中是很有前景的生物材料。本研究的目的是在体外研究碳掺杂二氧化硅纳米线（SiOC纳米线）对一种成纤维细胞系的细胞毒性。
通过化学气相沉积（CVD）工艺在硅基底上生长SiOC纳米线。将小鼠L929成纤维细胞培养在完全杜氏改良 Eagle 培养基（DMEM）中，并按照ISO 19003-5进行间接和直接细胞毒性试验，通过在MTT和化学发光测定中定量细胞活力来进行。在扫描电子显微镜（SEM）下观察细胞培养物，并通过免疫细胞化学来观察其形态并研究细胞与纳米线的相互作用。此外，通过SEM和酶联免疫吸附测定（ELISA）测定与富血小板血浆的血液相容性。
SiOC纳米线被证明具有生物相容性，并且在接触试验中不会损害细胞增殖。L929细胞能够附着在纳米线上并增殖。最有趣的是，L929细胞通过移动纳米结构并在纳米线网络内形成隧道来重组纳米线支架。此外，纳米线不会损害血小板活化，其表现与扁平二氧化硅相似。
我们的数据表明，SiOC纳米线不会释放细胞毒性物质，并且对成纤维细胞起到了可行且适应性良好的支架作用，因此代表了一种用于可植入装置的有前景的平台。
