Delpech N, Rapior S, Donnadieu P, Cozette A P, Ortiz J P, Huchard G
Laboratoire de Biochimie-Immunochimie, Centre Hospitalier Général, Perpignan.
Nephrologie. 1991;12(2):63-6.
Cortinarius poisoning is generally caused by orellanine, a hydroxy bipyridine N, N-dioxide. This intoxication is characterized by acute nephritis which can lead to death without treatment. We reported a highly sensitive and simple fluorimetric technique to analyse orellanine by thin-layer chromatography on the basis of its characteristic photodecomposition into orelline. Using this procedure, we detected and assayed orellanine for the first time in plasma and renal biopsies of a woman who had deliberately ingested two fruit-bodies of Cortinarius orellanus. An early original treatment was carried out based on hemodialysis, combination plasmapheresis-hemoperfusion, and amino acids and diltiazem administration. These results indicate that it is now possible to make a precise diagnosis of orellanine poisoning.
丝膜菌中毒通常由奥来毒素引起，奥来毒素是一种羟基联吡啶N，N - 二氧化物。这种中毒的特征是急性肾炎，如不治疗可导致死亡。我们报告了一种高度灵敏且简单的荧光技术，基于奥来毒素可光解为奥来酸的特性，通过薄层色谱法分析奥来毒素。使用该方法，我们首次在一名故意摄入两个奥来丝膜菌子实体的女性的血浆和肾活检组织中检测并测定了奥来毒素。基于血液透析、血浆置换 - 血液灌流联合治疗以及给予氨基酸和地尔硫䓬，进行了早期的原创性治疗。这些结果表明，现在有可能对奥来毒素中毒做出精确诊断。
