Department of Electrical and Computer Engineering, University of Connecticut, Storrs, CT 06269, USA.
Technol Cancer Res Treat. 2011 Oct;10(5):417-29. doi: 10.7785/tcrt.2012.500219.
We present an ultrasound (US)-guided diffuse optical tomography for mapping tumor deoxyhemoglobin (deoxyHb) and oxyhemoglobin (oxyHb) concentrations in blood phantoms and in in-vivo patients. Because oxyHb and deoxyHb respond differently at different wavelengths, four laser diodes of wavelengths 740 nm, 780 nm, 808 nm and 830 nm were used in the study. Tumor model experiments were performed using phantoms of different hemoglobin oxygen saturations (14%-89%) representing hemoglobin oxygenation in tissue. Targets of different sizes and located at different depths were used to validate the accuracy of oxygen saturation estimation. The absolute deviations between the estimated hemoglobin oxygen saturations obtained from reconstructed absorption maps and oxygen measurements obtained using a pO2 electrode were less than 8% over the measured range of oxygen saturation. An inhomogeneous concentric blood phantom of deoxygenated center core and oxygenated outer shell was imaged and deoxyHb and oxyHb maps revealed corresponding distributions which correlated well with inhomogeneous deoxy- and oxy- distributions frequently seen in breast cancers. Clinical examples are given to demonstrate the utility of US-guided optical tomography in mapping heterogeneous deoxyHb and oxyHb distributions in breast cancers.
我们提出了一种超声（US）引导的漫射光学层析成像技术，用于绘制血 phantom 和体内患者的肿瘤脱氧血红蛋白（deoxyHb）和氧合血红蛋白（oxyHb）浓度图。由于 oxyHb 和 deoxyHb 在不同波长下的反应不同，因此在研究中使用了四个波长为 740nm、780nm、808nm 和 830nm 的激光二极管。使用代表组织中血红蛋白氧合的不同血红蛋白氧饱和度（14%-89%）的 phantom 进行了肿瘤模型实验。使用不同大小和位于不同深度的目标验证了氧饱和度估计的准确性。从重建的吸收图中获得的估计血红蛋白氧饱和度与使用 pO2 电极获得的氧测量值之间的绝对偏差在测量的氧饱和度范围内小于 8%。对脱氧中心核和氧合外壳的不均匀同心血液 phantom 进行了成像，脱氧 Hb 和氧 Hb 图揭示了相应的分布，与乳腺癌中经常出现的不均匀脱氧和氧分布很好地相关。给出了临床示例，以证明 US 引导的光学层析成像在绘制乳腺癌中不均匀的脱氧 Hb 和氧 Hb 分布中的实用性。
