Slosar Magdalena, Vohra Poonam, Prasad Manju, Fischer Andrew, Quinlan Robert, Khan Ashraf
Department of Pathology, University of Massachusetts Medical School, UMass Memorial Medical Center, Three Biotech, One Innovation Drive, Worcester, MA 01605, USA.
Endocr Pathol. 2009 Fall;20(3):149-57. doi: 10.1007/s12022-009-9079-x.
Insulin-like growth factor mRNA binding protein 3 (IMP3) is an mRNA-binding protein that regulates transcription of insulin-like growth factor II affecting cell proliferation during embryogenesis. It is highly expressed in carcinomas of the pancreas, stomach, colon, rectum, kidneys, uterine cervix, lung, and ovary. The purpose of our study was to evaluate IMP3 expression in thyroid follicular lesions, to determine whether it has a role in differentiating among these lesions, and to understand their biological relationships. We immunostained 219 thyroid lesions selected from our surgical pathology archives including 14 hyperplastic colloid nodules (CN), 19 Hashimoto's thyroiditis (HT), two Graves disease (GD), ten Hürthle cell adenoma (HCA), 20 follicular adenoma (FA), 37 conventional papillary thyroid carcinoma (PTC), 60 follicular variant of papillary carcinoma (FVPC), 19 Hürthle cell carcinoma (HCC), 32 follicular carcinoma (FC), and six poorly differentiated/anaplastic carcinoma. Immunohistochemistry was performed on formalin-fixed sections using monoclonal antibody to IMP3. Clinicopathological data were also reviewed. In all cases, residual thyroid tissue, CN, HT, GD, HCA, and FA were completely negative for IMP3 staining. Of the 60 FVPC, 23 tumors (38%) were positive for IMP3, with 13 of these (22%) showing very strong staining (3+). Of the 32 FC, 22 tumors (69%) were positive, with seven (22%) showing very strong staining (3+). Furthermore, 33 out of 37 cases (89%) of PTC were negative for IMP3. In all four PTC cases that did stain positive, staining was weak-moderate (1-2+). Similarly, 15 out of 19 cases (79%) of HCC were negative. No significant correlation was found between pathologic tumor characteristics and IMP3 expression in differentiated follicular pattern thyroid carcinoma. With 100% specificity and 69% sensitivity for FC as compared to FA and 100% specificity for FVPC, again compared to FA, IMP3 has the potential to be diagnostically useful in differentiating malignant and benign follicular pattern thyroid lesions. This study also points to a possible common biological relationship between FC and FVPC that requires further investigation.
胰岛素样生长因子mRNA结合蛋白3（IMP3）是一种mRNA结合蛋白，它在胚胎发育过程中调节胰岛素样生长因子II的转录，影响细胞增殖。它在胰腺癌、胃癌、结肠癌、直肠癌、肾癌、子宫颈癌、肺癌和卵巢癌中高表达。我们研究的目的是评估IMP3在甲状腺滤泡性病变中的表达，确定其在鉴别这些病变中的作用，并了解它们之间的生物学关系。我们对从外科病理档案中选取的219例甲状腺病变进行免疫染色，包括14例增生性胶样结节（CN）、19例桥本甲状腺炎（HT）、2例格雷夫斯病（GD）、10例许特莱细胞腺瘤（HCA）、20例滤泡性腺瘤（FA）、37例传统乳头状甲状腺癌（PTC）、60例乳头状癌滤泡变体（FVPC）、19例许特莱细胞癌（HCC）、32例滤泡癌（FC）和6例低分化/未分化癌。使用抗IMP3单克隆抗体对福尔马林固定切片进行免疫组织化学检测。同时回顾临床病理资料。在所有病例中，残余甲状腺组织、CN、HT、GD、HCA和FA的IMP3染色均为阴性。在60例FVPC中，23例肿瘤（38%）IMP3呈阳性，其中13例（22%）呈强阳性染色（3+）。在32例FC中，22例肿瘤（69%）呈阳性，7例（22%）呈强阳性染色（3+）。此外，37例PTC中有33例（89%）IMP3为阴性。在所有4例IMP3染色阳性的PTC病例中，染色为弱阳性至中度（1-2+）。同样，19例HCC中有15例（79%）为阴性。在分化型滤泡状甲状腺癌中，病理肿瘤特征与IMP3表达之间未发现显著相关性。与FA相比，IMP3对FC的特异性为100%，敏感性为69%；与FA相比，对FVPC的特异性为100%，IMP3在鉴别甲状腺滤泡状恶性和良性病变方面具有诊断价值。本研究还指出FC和FVPC之间可能存在共同的生物学关系，需要进一步研究。
