Li Xiao-gang, Liu Biao, Liu Dou-dou, Han Zheng-min, Zhou Ke-xin, Zheng Yang-ping
College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, China.
Ying Yong Sheng Tai Xue Bao. 2009 Jan;20(1):157-62.
The decline of disease resistance of transgenic insect-resistant cotton has become one of the important factors restricting China cotton production. With two transgenic insect-resistant cotton varieties and their parental conventional cotton lines as test materials, the effects of their root exudates on the spore germination and mycelial growth of Verticillium dahliae Kleb were studied, and the components and contents of the amino acids and sugars in the exudates were determined. Comparing with their parental lines, the two transgenic insect-resistant varieties had a declined resistance against V. dahliae, and their root exudates had promotion effects on V. dahliae spore germination and mycelial growth. The root exudates of transgenic cotton Zhong-41, compared with those of its parent line Zhong-23, contained two additional amino acids Met and Lys, and the contents of Asp, Glu, Ser, Ala, Val, Leu, and Tyr were significantly higher. Though the components of amino acids in the root exudates of Simian-3 and transgenic cotton GK-12 were the same, the Tyr, Val, and Leu contents in the root exudates of transgenic cotton GK-12 were significantly lower. Four kinds of sugar were detected in the root exudates of Zhong-41, while only glucose was detected in the root exudates of Zhong-23. These four kinds of sugar were also detected in the root exudates of Simian-3 and GK12, but their contents differed significantly.
转基因抗虫棉抗病性下降已成为制约我国棉花生产的重要因素之一。以两个转基因抗虫棉品种及其亲本常规棉系为试验材料，研究了它们的根系分泌物对大丽轮枝菌孢子萌发和菌丝生长的影响，并测定了分泌物中氨基酸和糖类的成分及含量。与亲本相比，两个转基因抗虫品种对大丽轮枝菌的抗性下降，其根系分泌物对大丽轮枝菌孢子萌发和菌丝生长有促进作用。转基因棉花中41的根系分泌物与其亲本中23相比，含有额外的两种氨基酸甲硫氨酸和赖氨酸，天冬氨酸、谷氨酸、丝氨酸、丙氨酸、缬氨酸、亮氨酸和酪氨酸的含量显著更高。虽然泗棉3号和转基因棉花GK - 12根系分泌物中的氨基酸成分相同，但转基因棉花GK - 12根系分泌物中的酪氨酸、缬氨酸和亮氨酸含量显著更低。在中41的根系分泌物中检测到四种糖类，而在中23的根系分泌物中只检测到葡萄糖。在泗棉3号和GK12的根系分泌物中也检测到这四种糖类，但它们的含量差异显著。
