电脉冲作用下巨型囊泡收缩过程中膜损失的可视化。
Visualization of membrane loss during the shrinkage of giant vesicles under electropulsation.
作者信息
Portet Thomas, Camps i Febrer Franc, Escoffre Jean-Michel, Favard Cyril, Rols Marie-Pierre, Dean David S
机构信息
Institut de Pharmacologie et de Biologie Structurale, Centre National de la Recherche Scientifique, UMR 5089, Université Paul Sabatier, Toulouse, France.
出版信息
Biophys J. 2009 May 20;96(10):4109-21. doi: 10.1016/j.bpj.2009.02.063.
Abstract
We study the effect of permeabilizing electric fields applied to two different types of giant unilamellar vesicles, the first formed from EggPC lipids and the second formed from DOPC lipids. Experiments on vesicles of both lipid types show a decrease in vesicle radius, which is interpreted as being due to lipid loss during the permeabilization process. We show that the decrease in size can be qualitatively explained as a loss of lipid area, which is proportional to the area of the vesicle that is permeabilized. Three possible modes of membrane loss were directly observed: pore formation, vesicle formation, and tubule formation.
摘要
我们研究了施加于两种不同类型的巨型单层囊泡的通透电场的影响，第一种由蛋黄卵磷脂（EggPC）脂质形成，第二种由二油酰磷脂酰胆碱（DOPC）脂质形成。对这两种脂质类型的囊泡进行的实验均显示囊泡半径减小，这被解释为是由于通透过程中脂质损失所致。我们表明，尺寸的减小可以定性地解释为脂质面积的损失，其与被通透的囊泡面积成比例。直接观察到了三种可能的膜损失模式：孔形成、囊泡形成和小管形成。
相似文献
1
Visualization of membrane loss during the shrinkage of giant vesicles under electropulsation.电脉冲作用下巨型囊泡收缩过程中膜损失的可视化。
Biophys J. 2009 May 20;96(10):4109-21. doi: 10.1016/j.bpj.2009.02.063.
2
Lipid vesicles in pulsed electric fields: Fundamental principles of the membrane response and its biomedical applications.脉冲电场中的脂质体：膜响应的基本原理及其在生物医学中的应用。
Adv Colloid Interface Sci. 2017 Nov;249:248-271. doi: 10.1016/j.cis.2017.04.016. Epub 2017 Apr 28.
3
Asymmetric oxidation of giant vesicles triggers curvature-associated shape transition and permeabilization.巨泡的不对称氧化引发与曲率相关的形状转变和通透性。
Biophys J. 2009 Dec 2;97(11):2904-12. doi: 10.1016/j.bpj.2009.08.056.
4
Giant lipid vesicles under electric field pulses assessed by non invasive imaging.在电脉冲作用下的巨型脂质体的非侵入性成像评估。
Bioelectrochemistry. 2012 Oct;87:253-9. doi: 10.1016/j.bioelechem.2012.03.008. Epub 2012 Apr 5.
5
Electrostatic effects on the electrical tension-induced irreversible pore formation in giant unilamellar vesicles.静电效应对电张力诱导的巨大单层囊泡不可逆孔形成的影响。
Chem Phys Lipids. 2020 Sep;231:104935. doi: 10.1016/j.chemphyslip.2020.104935. Epub 2020 Jun 20.
6
Inward multivesiculation at the basal membrane of adherent giant phospholipid vesicles.贴壁巨型磷脂囊泡基底膜处的内向多泡形成。
Biochim Biophys Acta. 2016 Apr;1858(4):793-9. doi: 10.1016/j.bbamem.2016.01.027. Epub 2016 Jan 29.
7
Vesicle budding induced by a pore-forming peptide.由成孔肽诱导的小泡出芽。
J Am Chem Soc. 2010 Jan 13;132(1):195-201. doi: 10.1021/ja9059014.
8
Vesicle deformation and poration under strong dc electric fields.强直流电场下囊泡的变形与穿孔
Phys Rev E Stat Nonlin Soft Matter Phys. 2011 Jun;83(6 Pt 2):066316. doi: 10.1103/PhysRevE.83.066316. Epub 2011 Jun 21.
9
All-or-none versus graded: single-vesicle analysis reveals lipid composition effects on membrane permeabilization.全或无与分级：单囊泡分析揭示脂质组成对膜通透性的影响。
Biophys J. 2010 Dec 1;99(11):3619-28. doi: 10.1016/j.bpj.2010.09.027.
10
Prediction of the size of electroformed giant unilamellar vesicle using response surface methodology.使用响应面法预测电铸 giant unilamellar vesicle 的大小。
Biophys Chem. 2019 Oct;253:106217. doi: 10.1016/j.bpc.2019.106217. Epub 2019 Jul 4.
引用本文的文献
1
Synthesis Method and High Salt Concentration Can Affect Electrodeformation of GUVs under Strong Pulsed DC Fields.合成方法和高盐浓度会影响强脉冲直流电场下巨型单层囊泡的电极化。
ACS Omega. 2025 Feb 14;10(7):6427-6436. doi: 10.1021/acsomega.4c06412. eCollection 2025 Feb 25.
2
SMARTINI3 parametrization of multi-scale membrane models via unsupervised learning methods.通过无监督学习方法对多尺度膜模型进行 SMARTINI3 参数化。
Sci Rep. 2024 Oct 28;14(1):25714. doi: 10.1038/s41598-024-75490-2.
3
Probability and kinetics of rupture and electrofusion in giant unilamellar vesicles under various frequencies of direct current pulses.在不同频率的直流电脉冲下，巨单层囊泡破裂和电融合的概率和动力学。
PLoS One. 2024 Jun 10;19(6):e0304345. doi: 10.1371/journal.pone.0304345. eCollection 2024.
4
Assessing membrane material properties from the response of giant unilamellar vesicles to electric fields.通过巨型单层囊泡对电场的响应来评估膜材料特性。
Adv Phys X. 2023;8(1). doi: 10.1080/23746149.2022.2125342. Epub 2022 Oct 6.
5
All-atom molecular dynamics simulations of the combined effects of different phospholipids and cholesterol content on electroporation.不同磷脂和胆固醇含量对电穿孔联合作用的全原子分子动力学模拟
RSC Adv. 2022 Aug 30;12(38):24491-24500. doi: 10.1039/d2ra03895a.
6
High power electromagnetic pulse applicators for evaluation of biological effects induced by electromagnetic radiation waves.用于评估电磁辐射波诱导的生物效应的高功率电磁脉冲施加器。
RSC Adv. 2018 May 1;8(29):16319-16329. doi: 10.1039/c8ra00330k. eCollection 2018 Apr 27.
7
Juglone Inactivates through Cell Membrane Damage, Biofilm Blockage, and Inhibition of Gene Expression.胡桃醌通过破坏细胞膜、阻断生物膜和抑制基因表达来失活。
Molecules. 2021 Sep 27;26(19):5854. doi: 10.3390/molecules26195854.
8
Pulsed Electric Fields Promote Liposome Buddings.脉冲电场促进脂质体出芽。
Bioelectricity. 2021 Mar 1;3(1):68-76. doi: 10.1089/bioe.2020.0016. Epub 2021 Mar 16.
9
Response of an actin network in vesicles under electric pulses.囊泡中肌动蛋白网络对电脉冲的响应。
Sci Rep. 2019 May 31;9(1):8151. doi: 10.1038/s41598-019-44613-5.
10
The role of gel-phase domains in electroporation of vesicles.凝胶相结构域在囊泡电穿孔中的作用。
Sci Rep. 2018 Mar 19;8(1):4758. doi: 10.1038/s41598-018-23097-9.
本文引用的文献
1
Giant vesicles in electric fields.电场中的巨型囊泡
Soft Matter. 2007 Jun 19;3(7):817-827. doi: 10.1039/b703580b.
2
Membrane deformation under local pH gradient: mimicking mitochondrial cristae dynamics.局部pH梯度下的膜变形：模拟线粒体嵴动力学
Biophys J. 2008 Nov 15;95(10):4924-33. doi: 10.1529/biophysj.108.136077. Epub 2008 Aug 8.
3
Giant unilamellar vesicles containing phosphatidylinositol(4,5)bisphosphate: characterization and functionality.含有磷脂酰肌醇（4,5）二磷酸的巨型单层囊泡：表征与功能
Biophys J. 2008 Nov 1;95(9):4348-60. doi: 10.1529/biophysj.107.126912. Epub 2008 May 23.
4
Electrochemotherapy in treatment of tumours.电化学疗法治疗肿瘤
Eur J Surg Oncol. 2008 Feb;34(2):232-40. doi: 10.1016/j.ejso.2007.05.016. Epub 2007 Jul 5.
5
Membrane perturbation by an external electric field: a mechanism to permit molecular uptake.外部电场引起的膜扰动：一种允许分子摄取的机制。
Eur Biophys J. 2007 Nov;36(8):973-83. doi: 10.1007/s00249-007-0194-7. Epub 2007 Jun 19.
6
Electrotransfer as a non viral method of gene delivery.电穿孔转染作为一种非病毒基因递送方法。
Curr Gene Ther. 2007 Feb;7(1):67-77. doi: 10.2174/156652307779940207.
7
Transition from long- to short-lived transient pores in giant vesicles in an aqueous medium.在水介质中，巨型囊泡内从长寿命瞬态孔向短寿命瞬态孔的转变。
Phys Rev E Stat Nonlin Soft Matter Phys. 2006 Dec;74(6 Pt 1):061902. doi: 10.1103/PhysRevE.74.061902. Epub 2006 Dec 8.
8
Computer simulations of transport through membranes: passive diffusion, pores, channels and transporters.通过膜的转运的计算机模拟：被动扩散、孔道、通道和转运体
Clin Exp Pharmacol Physiol. 2006 Oct;33(10):893-903. doi: 10.1111/j.1440-1681.2006.04461.x.
9
Electric pulses induce cylindrical deformations on giant vesicles in salt solutions.电脉冲在盐溶液中的巨型囊泡上诱发圆柱形变形。
Biophys J. 2006 Sep 1;91(5):1778-86. doi: 10.1529/biophysj.106.081620. Epub 2006 Jun 9.
10
Free energy of a trans-membrane pore calculated from atomistic molecular dynamics simulations.通过原子分子动力学模拟计算得到的跨膜孔道自由能。
J Chem Phys. 2006 Apr 21;124(15):154905. doi: 10.1063/1.2171965.
Zotero 插件