Hiramitsu T, Armstrong D
Department of Ophthalmology, Hamamatsu University School of Medicine, Japan.
Ophthalmic Res. 1991;23(4):196-203. doi: 10.1159/000267103.
Lipid peroxidation was induced with porcine retinal homogenates using ferric iron or UV light. A number of lipid and aqueous-soluble antioxidants were tested and their protective effects measured after 60 min of exposure. The iron system produced up to 5 times more lipid hydroperoxides as quantified by the fluorescence thiobarbituric acid (TBA) assay. Of the three substituted dialkyl phenols evaluated, the most efficient was compound S-17224 which was able to afford greater than 90% protection at 10(-4) M concentration in the iron-induced system. At 10(-5) M, inhibition was 78% and at 10(-6) M 33%. By comparison, 10(-4) M gamma-oryzanol inhibited the reaction by 61% and alpha-tocopherol by only 14%. The combination of 10(-6) M S-17224 and 10(-4) M gamma-oryzanol potentiated the effect by another 23%. In the light-catalyzed system. S-17224 and alpha-tocopherol were more efficient (71%). The model system described here is simple and can be employed to study additional antioxidants as well as in the retinotoxic evaluation of ophthalmic drugs.
使用三价铁或紫外线诱导猪视网膜匀浆发生脂质过氧化。测试了多种脂溶性和水溶性抗氧化剂，并在暴露60分钟后测量了它们的保护作用。通过荧光硫代巴比妥酸（TBA）测定法量化，铁系统产生的脂质氢过氧化物比其他系统多5倍。在所评估的三种取代二烷基酚中，最有效的是化合物S-17224，在铁诱导系统中，其在10（-4）M浓度下能够提供大于90%的保护。在10（-5）M时，抑制率为78%，在10（-6）M时为33%。相比之下，10（-4）M的γ-谷维素抑制反应的比例为61%，α-生育酚仅为14%。10（-6）M的S-17224和10（-4）M的γ-谷维素联合使用可使效果再提高23%。在光催化系统中，S-17224和α-生育酚更有效（71%）。这里描述的模型系统很简单，可用于研究其他抗氧化剂以及眼科药物的视网膜毒性评估。
