Kamio A, Kummerow F A, Imai H
Arch Pathol Lab Med. 1977 Jul;101(7):378-81.
Twenty-eight weanling swine were divided into two groups and fed a basal ration of corn and soybean meal or one containing a high fat and cholesterol supplement. One subgroup of each received 220,000 IU of vitamin D3 per kilogram. Grossly normal areas of the distal abdominal aorta were used for quantitative comparison of degenerated smooth muscle cells by electron microscopical cell counts. Degeneration was classified into rarefaction, condensation, and frequency per 100 nucleated cells. The frequency of dead cells was demonstrable between the swine fed excess vitamin D3 with or without high fat and cholesterol and those fed the basal ration alone, or frequencies of 7.9, 7.4, and 5.6 at 3 months of age (P less than .05) and 7.3, 6.2, and 5.1 at 6 months (P less than .05), respectively. No significant difference was demonstrable between the swine fed high fat and cholesterol without excess vitamin D3 and those fed the basal ration alone, or frequencies of 5.7 and 5.6 at 3 months of age and 5.5 and 5.1 at 6 months.
将28只断奶仔猪分为两组，分别饲喂玉米和豆粕基础日粮或添加高脂肪和胆固醇补充剂的日粮。每组中的一个亚组每千克日粮添加22万国际单位的维生素D3。通过电子显微镜细胞计数，对腹主动脉远端大体正常区域的退化平滑肌细胞进行定量比较。退化分为核仁稀疏、核仁凝聚以及每100个有核细胞中的出现频率。在喂食过量维生素D3（无论是否添加高脂肪和胆固醇）的仔猪与仅喂食基础日粮的仔猪之间，可观察到死亡细胞的频率差异，3月龄时分别为7.9、7.4和5.6（P<0.05），6月龄时分别为7.3、6.2和5.1（P<0.05）。在喂食高脂肪和胆固醇但不过量维生素D3的仔猪与仅喂食基础日粮的仔猪之间，未观察到显著差异，3月龄时分别为5.7和5.6，6月龄时分别为5.5和5.1。
