Zakaria Z A, Sulaiman M R, Morsid N A, Aris A, Zainal H, Pojan N H Mohd, Kumar G Hanan
Pharmacogenomics Center, Faculty of Pharmacy, Universiti Teknologi MARA, Selangor, Malaysia.
Methods Find Exp Clin Pharmacol. 2009 Mar;31(2):81-8. doi: 10.1358/mf.2009.31.2.1353876.
The present study was carried out to evaluate the antinociceptive, anti-inflammatory and antipyretic effects of the aqueous extract of Solanum nigrum leaves using various animal models. The extract, at concentrations of 10, 50 and 100%, was prepared by soaking (1:20; w/v) air-dried powdered leaves (20 g) in distilled water (dH2O) for 72 h. The extract solutions were administered subcutaneously in mice/rats 30 min prior to the tests. The extract exhibited significant (P < 0.05) antinociceptive activity when assessed using the abdominal constriction, hot plate and formalin tests. The extract also produced significant (P < 0.05) anti-inflammatory and antipyretic activities when assessed using the carrageenan-induced paw edema and brewer's yeast-induced pyrexia tests, respectively. Overall, these activities occurred in a concentration-dependent manner, except for the 50% concentration of the extract, which was not effective in the abdominal constriction test. In conclusion, the present study demonstrated that S. nigrum leaves possessed antinociceptive, anti-inflammatory and antipyretic effects and thus supported traditional claims of its medicinal uses.
本研究旨在使用多种动物模型评估龙葵叶水提取物的抗伤害感受、抗炎和解热作用。通过将20克风干的叶粉（1:20；w/v）浸泡在蒸馏水中72小时，制备出浓度为10%、50%和100%的提取物。在测试前30分钟，将提取物溶液皮下注射到小鼠/大鼠体内。当使用腹部收缩、热板和福尔马林试验进行评估时，提取物表现出显著（P < 0.05）的抗伤害感受活性。当分别使用角叉菜胶诱导的爪肿胀和啤酒酵母诱导的发热试验进行评估时，提取物还产生了显著（P < 0.05）的抗炎和解热活性。总体而言，这些活性呈浓度依赖性，除了提取物的50%浓度在腹部收缩试验中无效。总之，本研究表明龙葵叶具有抗伤害感受、抗炎和解热作用，从而支持了其药用的传统说法。
