Zakaria Zainul Amiruddin, Gopalan Hanan Kumar, Zainal Hairani, Mohd Pojan Nur Hidayu, Morsid Nur Atiqah, Aris Anwariah, Sulaiman Mohd Roslan
Faculty of Biotechnology and Life Sciences, Universiti Industri Selangor, Jalan Zirkon A7/A, Seksyen 7, 40000 Shah Alam, Selangor, Malaysia.
Yakugaku Zasshi. 2006 Nov;126(11):1171-8. doi: 10.1248/yakushi.126.1171.
The present study was carried out to evaluate the antinociceptive, anti-inflammatory and antipyretic effects of chloroform extract of Solanum nigrum leaves using various animal models.
The extract was prepared by soaking (1:20; w/v) the air-dried powdered leaves (20 g) in chloroform for 72 hrs followed by evaporation (40 degrees C) under reduced pressure to dryness (1.26 g) and then dissolved (1:50; w/v) in dimethylsulfoxide (DMSO). The supernatant, considered as the stock solution with dose of 200 mg/kg, was diluted using DMSO to 20 and 100 mg/kg, and all doses were administered (s.c.; 10 ml/kg) in mice/rats 30 min prior to tests.
The extract exhibited significant (p<0.05) antinociceptive activity when assessed using the abdominal constriction, hot plate and formalin tests. The extract also produced significant (p<0.05) anti-inflammatory and antipyretic activities when assessed using the carrageenan-induced paw edema and brewer's yeast-induced pyrexia tests. Overall, the activities occurred in a dose-independent manner.
The present study demonstrated that the lipid-soluble extract of S. nigrum leaves possessed antinociceptive, anti-inflammatory and anti-pyretic properties and confirmed the traditional claims.
本研究旨在使用多种动物模型评估龙葵叶氯仿提取物的抗伤害感受、抗炎和解热作用。
将风干的粉末状叶子（20克）以1:20（重量/体积）的比例浸泡在氯仿中72小时，然后在40摄氏度减压蒸发至干（1.26克），接着以1:50（重量/体积）的比例溶解于二甲基亚砜（DMSO）中。将上清液视为剂量为200毫克/千克的储备溶液，用DMSO稀释至20和100毫克/千克，所有剂量均在测试前30分钟腹腔注射（10毫升/千克）给小鼠/大鼠。
当使用腹部收缩、热板和福尔马林试验评估时，提取物表现出显著（p<0.05）的抗伤害感受活性。当使用角叉菜胶诱导的爪肿胀和啤酒酵母诱导的发热试验评估时，提取物还产生了显著（p<0.05）的抗炎和解热活性。总体而言，这些活性呈剂量非依赖性。
本研究表明龙葵叶的脂溶性提取物具有抗伤害感受、抗炎和解热特性，并证实了传统说法。
