Castel-Branco M M, Alves G L, Figueiredo I V, Falcão A C, Caramona M M
Laboratory of Pharmacology, Faculty of Pharmacy, Coimbra University, Coimbra, Portugal.
Methods Find Exp Clin Pharmacol. 2009 Mar;31(2):101-6. doi: 10.1358/mf.2009.31.2.1338414.
The choice of appropriate animal models for the initial in vivo testing of potential anticonvulsant compounds is one of the most important steps in the successful search for new antiepileptic drugs. The purpose of this paper is to describe the most important aspects to take into account when performing the maximal electroshock seizure (MES) test in the routine laboratory screening of new antiepileptics: the conventional and threshold MES test experimental procedures, the factors affecting experimental data (laboratory conditions, administration vehicles and drug formulations, time after drug administration, and stimulus duration and site of stimulation) and the assessment of anticonvulsant activity are discussed.
为潜在抗惊厥化合物进行初步体内测试选择合适的动物模型，是成功寻找新型抗癫痫药物过程中最重要的步骤之一。本文旨在描述在新抗癫痫药物常规实验室筛选中进行最大电休克惊厥（MES）测试时需要考虑的最重要方面：讨论了常规和阈值MES测试的实验程序、影响实验数据的因素（实验室条件、给药载体和药物制剂、给药后时间、刺激持续时间和刺激部位）以及抗惊厥活性评估。
