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微机电系统材料对 PCR 产量的影响。

Effect of materials for micro-electro-mechanical systems on PCR yield.

机构信息

Fondazione Bruno Kessler (FBK), Center for Materials and Microsystems, via Sommarive 18, Povo (Trento), Italy.

出版信息

Eur Biophys J. 2010 May;39(6):979-86. doi: 10.1007/s00249-009-0466-5. Epub 2009 May 20.

Abstract

In this study we analyzed the surface properties of different silicon-based materials used for micro-electro-mechanical systems (MEMS) production, such as thermally grown silicon oxide, plasma-enhanced chemical vapor deposition (PECVD)-treated silicon oxide, reactive-ion etch (RIE)-treated silicon oxide, and Pyrex. Substrates were characterized by atomic force microscopy (AFM) and X-ray photoelectron spectroscopy (XPS) to define the surface chemical and morphological properties, and by fluorescence microscopy to directly assess the absorption of the different polymerase chain reaction (PCR) components. By using microchips fabricated with the same materials we investigated their compatibility with PCR reactions, exploiting the use of different enzymes and reagents or proper surface treatments. We established the best conditions for DNA amplification in silicon/Pyrex microdevices depending on the type of device and fabrication method used and the quality of reagents, rather than on the passivation treatment or increment in standard Taq polymerase concentration.

摘要

在这项研究中,我们分析了用于微机电系统(MEMS)生产的不同硅基材料的表面特性,如热生长氧化硅、等离子体增强化学气相沉积(PECVD)处理氧化硅、反应离子刻蚀(RIE)处理氧化硅和 Pyrex。通过原子力显微镜(AFM)和 X 射线光电子能谱(XPS)对基底进行特性描述,以确定表面化学和形态特性,并通过荧光显微镜直接评估不同聚合酶链反应(PCR)成分的吸收情况。通过使用相同材料制造的微芯片,我们研究了它们与 PCR 反应的兼容性,利用了不同酶和试剂或适当的表面处理。我们根据器件的类型和制造方法以及试剂的质量,而不是基于钝化处理或标准 Taq 聚合酶浓度的增加,确定了在硅/Pyrex 微器件中进行 DNA 扩增的最佳条件。

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