Faure J P, de Kozak Y, Dorey C, Tuyen V V
Arch Ophtalmol (Paris). 1977;37(1):47-60.
24 different antigenic preparations from bovine or guinea pig retina and 3 from bovine uvea were tested for their ability to induce uveo-retinitis in guinea pigs. Each animal received one injection into the hind foot pads of 0.1 ml og the tissue preparation mixed with an equal volume of complete Freund's adjuvant. The intensity of the disease was assessed by clinical and histological criteria. Homogenates and extracts from whole guinea pig retina are more active than the same preparations from bovine retina. Autologous retinal extract is slightly more active than homologous in low doses. In bovine retina, the autoantigen(s) is localized in the photoreceptor structures and the pigment epithelium. Bovine uveal preparations seem to be inactive when the epithelium has been removed. Purified outer segments are very active, as well as soluble extracts of outer segments. Highly purified bovine rhodopsin has no immunopathogenic activity. A soluble autoantigen (autoantigen S) has been isolated by preparative isoelectrofocusing from retinas of several species. Autoantigen S from guinea pig induces the disease in guinea pigs at a dose of a few micrograms.
对来自牛或豚鼠视网膜的24种不同抗原制剂以及来自牛葡萄膜的3种抗原制剂进行了测试，以评估它们在豚鼠中诱发葡萄膜视网膜炎的能力。每只动物在后足垫注射0.1毫升与等量完全弗氏佐剂混合的组织制剂。通过临床和组织学标准评估疾病的严重程度。豚鼠全视网膜的匀浆和提取物比牛视网膜的相同制剂更具活性。低剂量时，自体视网膜提取物比同种异体提取物的活性略高。在牛视网膜中，自身抗原定位于光感受器结构和色素上皮。去除上皮后，牛葡萄膜制剂似乎无活性。纯化的外段以及外段的可溶性提取物非常活跃。高度纯化的牛视紫红质没有免疫致病活性。通过制备性等电聚焦从几种物种的视网膜中分离出一种可溶性自身抗原（自身抗原S）。豚鼠的自身抗原S以几微克的剂量可在豚鼠中诱发疾病。
