Division of Craniofacial Function Engineering, Tohoku University Graduate School of Dentistry, Aoba-ku, Sendai, Japan.
Tissue Eng Part A. 2009 Nov;15(11):3525-35. doi: 10.1089/ten.TEA.2009.0048.
The present study was designed to investigate whether octacalcium phosphate (OCP)-precipitated alginate (Alg) promotes osteoblastic cell proliferation and bone regeneration in comparison with Alg itself. Alg, known to lack mammal cell attachment capability, was used as the matrix to test the distribution effect of OCP in a three-dimensional environment. A series of Alg/OCP scaffolds with different pore sizes was prepared by centrifuging Alg gels precipitated by OCP crystals. The scaffolds had a bimodal distribution of pores (ultrafine pores: approximately 100 nm; relatively large pores: from 6.0 to 51.7 microm) and over 86% porosity. The osteoconductive capability of Alg/OCP was determined by examining mouse bone marrow stromal ST-2 cell proliferation after 3 days in vitro and bone regeneration in mouse calvaria critical-sized defect after 21 days. The analyses showed that ST-2 cell proliferation and bone regeneration increased with an increase in the pore size and reached the highest level in the 51.7 microm pore scaffold. The results suggest that OCP-precipitated Alg provides a better scaffold for osteoblasts to attach and proliferate in a three-dimensional environment and promotes bone regeneration, indicating that OCP is a candidate material to modify the surface of non-cell-interactive polymeric scaffolds, such as Alg, into an osteogenic condition.
本研究旨在探讨与藻酸盐（Alg）本身相比，八钙磷酸盐（OCP）沉淀的藻酸盐（Alg）是否能促进成骨细胞增殖和骨再生。Alg 作为基质，已知缺乏哺乳动物细胞附着能力，用于测试 OCP 在三维环境中的分布效果。通过离心 OCP 晶体沉淀的 Alg 凝胶，制备了一系列具有不同孔径的 Alg/OCP 支架。支架具有双模态孔径分布（超细孔：约 100nm；较大孔：6.0 至 51.7μm）和超过 86%的孔隙率。通过检测体外 3 天小鼠骨髓基质 ST-2 细胞增殖和 21 天小鼠颅骨临界大小缺损的骨再生，确定了 Alg/OCP 的骨诱导能力。分析表明，ST-2 细胞增殖和骨再生随孔径增大而增加，在 51.7μm 孔支架中达到最高水平。结果表明，OCP 沉淀的 Alg 为成骨细胞在三维环境中附着和增殖提供了更好的支架，并促进了骨再生，表明 OCP 是一种候选材料，可以将非细胞相互作用的聚合物支架（如 Alg）的表面改性为成骨性条件。
