Division of Advanced Prosthetic Dentistry, Tohoku University Graduate School of Dentistry, 4-1 Seiryo-machi, Aoba-ku, Sendai, 980-8575, Japan.
J Mater Sci Mater Med. 2010 Mar;21(3):907-14. doi: 10.1007/s10856-009-3911-1. Epub 2009 Oct 23.
The present study was designed to investigate whether the amount of octacalcium phosphate (OCP) affects the characteristics of alginate (Alg)/OCP scaffolds regarding the pore formation and its distribution, and the thermodynamic stability from OCP to hydroxyapatite (HA) in an in vitro physiological environment. Alg/OCP composites with weight ratios of 100/0, 75/25, 50/50, and 25/75 were prepared through mixing the ground synthesized OCP crystals with an Alg solution and applying lyophilization. Analysis of X-ray diffraction, Fourier transform infrared (FTIR) spectroscopy, and mercury intrusion porosimetry verified that the crystalline phase of OCP and the porosities were retained regardless of the OCP amount. On the other hand, the elastic modulus, determined by mechanical testing, and, interestingly, the pore size increased with increasing the OCP amount. The immersion of the composites in a simulated body fluid up to 14 days revealed that OCP in Alg matrices tends to convert to HA with enhancing the calcium consumption depending on the OCP amount. The results indicated that the inclusion of OCP crystals in the Alg matrix by the mixing process controls the character of the pore distribution in Alg/OCP composites while maintaining the transitory nature of OCP.
本研究旨在探讨八钙磷灰石（OCP）的含量是否会影响藻酸盐（Alg）/OCP 支架的特性，包括在体外生理环境中孔形成及其分布，以及从 OCP 到羟基磷灰石（HA）的热力学稳定性。通过将合成的 OCP 晶体与 Alg 溶液混合并进行冷冻干燥，制备了重量比为 100/0、75/25、50/50 和 25/75 的 Alg/OCP 复合材料。X 射线衍射、傅里叶变换红外（FTIR）光谱和压汞法分析证实，OCP 的晶相和孔隙率保持不变，与 OCP 的含量无关。另一方面，通过机械测试确定的弹性模量，以及有趣的是，孔径随 OCP 含量的增加而增大。将复合材料在模拟体液中浸泡 14 天表明，Alg 基质中的 OCP 倾向于根据 OCP 的含量转化为 HA，同时消耗钙。结果表明，通过混合过程将 OCP 晶体纳入 Alg 基质可以控制 Alg/OCP 复合材料中孔分布的特征，同时保持 OCP 的短暂性质。
