Ferrandino Ida, Favorito Rossana, Annunziata Martina, Grimaldi Maria Consiglio
Department of Biological Sciences, Section of Evolutionary and Comparative Biology, University Federico II of Naples, Naples, Italy.
Ann N Y Acad Sci. 2009 Apr;1163:386-8. doi: 10.1111/j.1749-6632.2008.03667.x.
The cytotoxic effects of cadmium (Cd) on the pituitary gland were studied in the lizard Podarcis sicula. Adult lizards were treated intraperitoneally with a single injection of CdCl(2) at the dose of 2 mg/kg. A morphological study was performed by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) technique and by immunocytochemical demonstration of caspase-3 for detecting apoptotic cells in situ after 2, 7, and 16 days of treatment. The pituitary cells of Cd-treated P. sicula undergo apoptosis: after 2 days the apoptotic cells appeared to increase significantly compared with those of the control specimens; after 7 days there was no considerable increment; however, after 16 days the occurrence of apoptotic cells had increased markedly, above all in the rostral pars distalis (RPD) in which various cells showed a strongly immunostained nucleus by TUNEL. Cd appears particularly toxic for pituitary cells in the lizard P. sicula; a single high intraperitoneal dose of CdCl(2) induces apoptosis, in particular in the RPD, and this effect appears irreversible.
研究了镉（Cd）对意大利壁蜥垂体的细胞毒性作用。成年蜥蜴腹腔注射一次剂量为2 mg/kg的CdCl₂。通过末端脱氧核苷酸转移酶介导的dUTP缺口末端标记（TUNEL）技术和caspase-3免疫细胞化学检测，在处理2、7和16天后原位检测凋亡细胞，进行形态学研究。经镉处理的意大利壁蜥垂体细胞发生凋亡：处理2天后，凋亡细胞与对照标本相比显著增加；7天后没有显著增加；然而，16天后凋亡细胞的发生率显著增加，尤其是在吻部远侧部（RPD），其中各种细胞经TUNEL检测显示细胞核强烈免疫染色。镉对意大利壁蜥的垂体细胞似乎具有特别的毒性；单次腹腔注射高剂量的CdCl₂可诱导凋亡，尤其是在RPD，并且这种效应似乎是不可逆的。
