利用SRAP对不同产地蒙古黄芪遗传关系的研究
[Study on genetic relationship of Astragalus membranaceus var mongholicus in different producing area using SRAP].
作者信息
Qian Dan, Huang Luqi, Cui Guanghong, Chen Min
机构信息
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2009 Feb;34(4):382-5.
OBJECTIVE
To study the genetic relationship of Astragalus membranaceus var. mongholicus in different producing area and provide theoretical basis for the evaluation of Astragalus germplasm resources.
METHOD
Through quence-related amplified polymorphism (SRAP) analysis, the systematic diagram of genetic relationship was constructed by UPGMA method.
RESULT
A total of 141 SRAP markers were scored. By the use of UPGMA cluster analysis with genetic distance, Astragalus could be divided into two provenance plots of Gansu and Shanxi.
CONCLUSION
The genetic differentiation among populations of A. membranaceus var. mongholicus is remarkable. SRAP marker could be efficiently used for the study of the genetic relationship of Astragalus.
目的
研究不同产地蒙古黄芪的亲缘关系，为黄芪种质资源评价提供理论依据。
方法
采用序列相关扩增多态性（SRAP）分析，通过非加权组平均法（UPGMA）构建亲缘关系系统图。
结果
共获得141个SRAP标记。利用遗传距离进行UPGMA聚类分析，黄芪可分为甘肃和山西两个产地类群。
结论
蒙古黄芪居群间遗传分化显著。SRAP标记可有效用于黄芪亲缘关系研究。
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