人参皂苷Rg1对神经干细胞的体外增殖作用

[Proliferation effect of neural stem cell of ginsenoside Rg1 in vitro].

作者信息

Zhuang Pengwei, Zhang Yanjun, Pang Tan

机构信息

Tianjin University of Traditional Chinese Medicine, Tianjin Laboratory of Chinese Medience Pharmacology, Tianjin 300193, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2009 Feb;34(4):443-6.

PMID:19459308

Abstract

OBJECTIVE

To study the proliferation effect of neural stem cells (NSCs) of ginsenoside Rg1 in vitro.

METHOD

NSCs from the embryonic rat (E14) were isolated, 10 mg x L(-1) BrdU were added in the medium. The NSCs were incubated together with 1, 10 micromol x L(-1) ginsenoside Rg1 for 48 hours, control group were setup. Then BrdU positive cell number were detected and counted by fluorescence microscop. The Hes1 and Mash1 mRNA expression were detected by real-time RT-PCR.

RESULT

The number of BrdU positive cells in 10 micromol x L(-1) ginsenoside Rg1 group was increased significantly compared with the control group (40.5 +/- 10.9 vs 26.2 +/- 6.0, P < 0.01), and the Hes1 mRNA expression were increased significantly (1.00 +/- 0.11) vs (1.28 +/- 0.14), P < 0.05.

CONCLUSION

ginsenoside Rg1 can promote the proliferation of neural stem cells in vitro, and this effect may be induced by the up-regulation Hes1 expression.

摘要

目的

研究人参皂苷Rg1对神经干细胞（NSCs）的体外增殖作用。

方法

分离胚胎大鼠（E14）的神经干细胞，培养基中加入10 mg·L⁻¹溴脱氧尿苷（BrdU）。将神经干细胞与1、10 μmol·L⁻¹人参皂苷Rg1共同孵育48小时，设立对照组。然后通过荧光显微镜检测并计数BrdU阳性细胞数量。通过实时逆转录聚合酶链反应（RT-PCR）检测Hes1和Mash1 mRNA表达。