黄芩苷和人参皂苷Rb1促进阿尔茨海默病模型大鼠神经干细胞的增殖和分化。
Baicalin and ginsenoside Rb1 promote the proliferation and differentiation of neural stem cells in Alzheimer's disease model rats.
作者信息
Zhao Jiwei, Lu Shanshan, Yu Hongli, Duan Shurong, Zhao Jingkun
机构信息
Department of Neurology, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, China.
Department of Neurology, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, China.
出版信息
Brain Res. 2018 Jan 1;1678:187-194. doi: 10.1016/j.brainres.2017.10.003. Epub 2017 Oct 14.
BACKGROUND
This study aimed to explore the effects of ginsenoside Rb1 and baicalin on the proliferation and differentiation of neural stem cells (NSC) in Alzheimer's disease model rats.
METHOD
The healthy Sprague Dawley male rats were randomly divided into 4 groups: control group, model group, ginsenoside Rb1 group and baicalin group. Besides, the animal model of dementia was induced by the injection of Aβ1-40. 2 weeks later, the rats in the baicalin and ginsenoside Rb1 groups were injected with baicalin and ginsenoside Rb1, respectively. The contents, expression sites of Nestin, GFAP and NSE and the percentage of viable cells were detected by immunohistochemistry. In addition, the expression levels of Nestin, GFAP and NSE in hippocampus of rats were detected by western-blot and metrology analysis was performed using quantity.
RESULTS
Injection of Aβ1-40 significantly reduced the number of neuronal cells (p < .05). In addition, compared with the control group, the percentages of positive cells of NSCs, astrocytes and neuronal were increased. Besides, compared with the model group, the percentage of positive neural cells was improved by ginsenoside Rb1 (p < .05), and the percentages of astrocytes and neuronal were increased by ginsenoside Rb1 and baicalin (p < .05). Moreover, the expressions of Nestin and NSE were enhanced by ginsenoside Rb1 and baicalin (p < .05), while the GFAP level was only affected by ginsenoside Rb1 (p < .05) when compared with the model group.
CONCLUSION
Ginsenoside Rb1 and baicalin might promote the proliferation and differentiation of endogenous NSCs in AD rat model.
背景
本研究旨在探讨人参皂苷Rb1和黄芩苷对阿尔茨海默病模型大鼠神经干细胞（NSC）增殖和分化的影响。
方法
将健康的Sprague Dawley雄性大鼠随机分为4组：对照组、模型组、人参皂苷Rb1组和黄芩苷组。此外，通过注射Aβ1-40诱导痴呆动物模型。2周后，黄芩苷组和人参皂苷Rb1组大鼠分别注射黄芩苷和人参皂苷Rb1。采用免疫组织化学法检测巢蛋白（Nestin）、胶质纤维酸性蛋白（GFAP）和神经元特异性烯醇化酶（NSE）的含量、表达部位及活细胞百分比。此外，采用蛋白质免疫印迹法检测大鼠海马中Nestin、GFAP和NSE的表达水平，并进行定量计量分析。
结果
注射Aβ1-40可显著减少神经元细胞数量（p<0.05）。此外，与对照组相比，神经干细胞、星形胶质细胞和神经元的阳性细胞百分比增加。此外，与模型组相比，人参皂苷Rb1可提高神经阳性细胞百分比（p<0.05），人参皂苷Rb1和黄芩苷可增加星形胶质细胞和神经元的百分比（p<0.05）。此外，与模型组相比，人参皂苷Rb1和黄芩苷可增强Nestin和NSE的表达（p<0.05），而GFAP水平仅受人参皂苷Rb1影响（p<0.05）。
结论
人参皂苷Rb1和黄芩苷可能促进AD大鼠模型中内源性神经干细胞的增殖和分化。
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