Radko A, Słota E
National Research Institute of Animal Production, Department of Immuno- and Cytogenetics, Krakowska 1, 32-083 Balice, Poland.
Pol J Vet Sci. 2009;12(1):113-7.
The aim of this study was to analyse the polymorphism of 19 microsatellite markers and their usefulness for parentage verification in 28 Borzoi dogs in Poland. The assay involved the following 19 loci: AHTk211, CXX279, REN169018, INU055, REN54P11, INRA21, AHT137, REN169D01, AHTh260, AHTk253, INU005, INU030, FH2848, AHT121, FH2054, REN162C04, AHThl71, REN247M23 and amelogenin. These markers are included in the panel of loci recommended by the ISAG. Amplified PCR products were analysed by an automated sequencer ABI PRISM 3130xl Genetic Analyser. The number of alleles per locus varied from 3 (INU005) to 8 (REN162 and AHT171). Except the INU005 locus, which showed the lowest variation (PIC=0.396, H=0.438), the calculated PIC values exceeded 0.6 and H values ranged from 0.677 (INU055) to as much as 0.84 (REN162). Based on PE(C) it was found that incorrect pedigree in this breed can be excluded with 99.9998% accuracy using DNA analysis.
本研究的目的是分析19个微卫星标记的多态性及其在波兰28只波索尔犬亲子关系鉴定中的效用。该检测涉及以下19个位点：AHTk211、CXX279、REN169018、INU055、REN54P11、INRA21、AHT137、REN169D01、AHTh260、AHTk253、INU005、INU030、FH2848、AHT121、FH2054、REN162C04、AHThl71、REN247M23和牙釉蛋白。这些标记包含在国际动物遗传学会推荐的位点组中。扩增的PCR产物通过自动测序仪ABI PRISM 3130xl遗传分析仪进行分析。每个位点的等位基因数量从3个（INU005）到8个（REN162和AHT171）不等。除显示出最低变异的INU005位点（PIC = 0.396，H = 0.438）外，计算出的PIC值超过0.6，H值范围从0.677（INU055）到高达0.84（REN162）。基于PE(C)发现，使用DNA分析可以以99.9998%的准确率排除该品种中不正确的谱系。
