Department of Animal Pathology, University of Pisa, Italy.
Anim Genet. 2012 Aug;43(4):463-7. doi: 10.1111/j.1365-2052.2011.02267.x. Epub 2011 Oct 18.
Eight short tandem repeat markers included in the International Society for Animal Genetics panel of 24 loci investigated in canine comparison tests were analysed in a sample of pure-breed dogs, with the purpose of defining an allele nomenclature based on the number of repeats. A regression analysis of the raw data produced by the sequencer, coupled with the direct sequencing of selected alleles, allowed us to propose a system of nomenclature for six of the eight loci (four di-nucleotidic: AHT121, AHTh137, REN169018 and REN64E19, and two tetra-nucleotidic: FH2001 and FH2328). The remaining two loci (INU055 and FH2848) showed a pattern of fragments that did not resolve in a simple allele series. This work may be useful to establish a basis for comparing data across different laboratories for a set of validated canine markers, which can be used in population genetics, forensics and other analyses.
八个短串联重复标记物包含在国际动物遗传学会的 24 个犬种比较测试的标记物中，在纯种犬样本中进行了分析，目的是根据重复次数定义等位基因命名法。测序仪产生的原始数据的回归分析，加上对选定等位基因的直接测序，使我们能够为其中的六个位点（四个二核苷酸：AHT121、AHTh137、REN169018 和 REN64E19，两个四核苷酸：FH2001 和 FH2328）提出一个命名系统。其余两个位点（INU055 和 FH2848）显示出的片段模式不能简单地解析为等位基因系列。这项工作对于为一组经过验证的犬类标记物在不同实验室之间比较数据建立基础可能是有用的，这些标记物可用于群体遗传学、法医学和其他分析。
