Medvedev B I, Teplova S N, Zaĭnetdinova L F
Zh Mikrobiol Epidemiol Immunobiol. 2009 Mar-Apr(2):80-5.
Optimization of diagnostics of genital herpesvirus infection (herpes simplex virus types 1 and 2, cytomegalovirus [CMV]) in women with tubal-peritoneal infertility on the basis of detection of viral material in various parts of genital system using polymerase chain reaction (PCR) and revealing pathogenetically significant systemic abnormalitites of cytokine- and nitroxidergic regulation.
Sixty-three women with tubal-peritoneal infertility were included in the study. Immunoenzyme assay, PCR, and modified Griess method were used.
In cervical canal herpesvirus infection was detected together with other agents in 30.1% of cases. In upper parts of reproductive system monoinfection with herpesvirus was detected in 74.63% of cases (herpes virus types 1 and 2--in 46.03%, CMV--in 28.6% of women). Increased levels of stable end metabolites of nitric oxide and cytokines were noted in sera of tested women.
Inflammatory changes in reproductive organs in women with tubal-peritoneal infertility are determined by chronic herpesvirus infection. This leads to Th1 polarization of immune response and developing of immune-mediated inflammation.
基于聚合酶链反应（PCR）检测生殖系统各部位的病毒物质，并揭示细胞因子和一氧化氮调节在发病机制上的显著系统性异常，优化对输卵管 - 腹膜性不孕女性生殖器疱疹病毒感染（1型和2型单纯疱疹病毒、巨细胞病毒[CMV]）的诊断。
63例输卵管 - 腹膜性不孕女性纳入研究。采用免疫酶测定法、PCR和改良格里斯方法。
在30.1%的病例中，宫颈管疱疹病毒感染与其他病原体同时被检测到。在生殖系统上部，74.63%的病例检测到单纯疱疹病毒感染（1型和2型疱疹病毒感染占46.03%，CMV感染占28.6%的女性）。检测女性血清中一氧化氮和细胞因子的稳定终末代谢产物水平升高。
输卵管 - 腹膜性不孕女性生殖器官的炎症变化由慢性疱疹病毒感染决定。这导致免疫反应的Th1极化和免疫介导炎症的发展。
