Park Min Ju, Lee Eun Kyeong, Heo Hyoung-Sam, Kim Min-Sun, Sung Bokyoung, Kim Mi Kyung, Lee Jaewon, Kim Nam Deuk, Anton Stephen, Choi Jae Sue, Yu Byung Pal, Chung Hae Young
Department of Pharmacy, College of Pharmacy, Pusan National University, Busan, Republic of Korea.
J Med Food. 2009 Apr;12(2):351-8. doi: 10.1089/jmf.2008.0006.
Kaempferol, one of the phytoestrogens, is found in berries and Brassica and Allium species and is known to have antioxidative and anti-inflammatory properties. In the present study, we examined the molecular mechanisms underlying the anti-inflammation effect of kaempferol in an aged animal model. To examine the effect of kaempferol in aged Sprague-Dawley rats, kaempferol was fed at 2 or 4 mg/kg/day for 10 days. The data show that kaempferol exhibited the ability to maintain redox balance. Kaempferol suppressed nuclear factor-kappaB (NF-kappaB) activation and expression of its target genes cyclooxygenase-2, inducible nitric oxide synthase, monocyte chemoattractant protein-1, and regulated upon activation, and normal T-cell expressed and secreted in aged rat kidney and in tert-butylhydroperoxide-induced YPEN-1 cells. Furthermore, kaempferol suppressed the increase of the pro-inflammatory NF-kappaB cascade through modulation of nuclear factor-inducing kinase (NIK)/IkappaB kinase (IKK) and mitogen-activated protein kinases (MAPKs) in aged rat kidney. Based on these results, we concluded that anti-oxidative kaempferol suppressed the activation of inflammatory NF-kappaB transcription factor through NIK/IKK and MAPKs in aged rat kidney.
山奈酚是一种植物雌激素，存在于浆果、十字花科植物和葱属植物中，已知具有抗氧化和抗炎特性。在本研究中，我们研究了山奈酚在老年动物模型中抗炎作用的分子机制。为了研究山奈酚对老年斯普拉格-道利大鼠的影响，以2或4mg/kg/天的剂量给大鼠喂食山奈酚，持续10天。数据表明，山奈酚具有维持氧化还原平衡的能力。山奈酚抑制了核因子-κB（NF-κB）的激活及其靶基因环氧合酶-2、诱导型一氧化氮合酶、单核细胞趋化蛋白-1的表达，并在老年大鼠肾脏以及叔丁基过氧化氢诱导的YPEN-1细胞中调节激活后正常T细胞表达和分泌。此外，山奈酚通过调节老年大鼠肾脏中的核因子诱导激酶（NIK）/IκB激酶（IKK）和丝裂原活化蛋白激酶（MAPK）来抑制促炎NF-κB级联反应的增加。基于这些结果，我们得出结论，抗氧化的山奈酚通过老年大鼠肾脏中的NIK/IKK和MAPK抑制炎性NF-κB转录因子的激活。
