Vanhoutvin S A L W, Troost F J, Kilkens T O C, Lindsey P J, Hamer H M, Jonkers D M A E, Venema K, Brummer R-J M
TI Food and Nutrition, Wageningen, The Netherlands.
Neurogastroenterol Motil. 2009 Sep;21(9):952-e76. doi: 10.1111/j.1365-2982.2009.01324.x. Epub 2009 May 19.
Fermentation of dietary fibres by colonic microbes leads to the production of short chain fatty acids (mainly propionate, butyrate and acetate), which are utilized by the colonic mucosa. Previous studies showed positive effects of butyrate on parameters of oxidative stress, inflammation and apoptosis. Recent studies in rats, however, showed that butyrate increased visceral sensitivity. The aim of this study was to determine the effects of physiologically relevant concentrations of butyrate on visceral perception in healthy human subjects. Eleven healthy volunteers participated in this randomized double-blind, placebo controlled cross-over study. The study consisted of three periods of 1 week each, in which the volunteers daily self-administered rectal enemas containing 100, 50 mmol L(-1) butyrate, or placebo (saline) prior to sleeping. A rectal barostat measurement was performed at the start and the end of each test period for the measurement of pain, urge and discomfort. Butyrate treatment resulted in a dose-dependent reduction of pain, urge and discomfort throughout the entire pressure range of the protocol. At a pressure of 4 mmHg, 50 and 100 mmol L(-1) butyrate concentrations resulted in a 23.9% and 42.1% reduction of pain scores, respectively, and the discomfort scores decreased by 44.2% and 69.0% respectively. At a pressure of 67 mmHg, 50 and 100 mmol L(-1) of butyrate decreased the pain scores by 23.8% and 42%, respectively, and discomfort scores 1.9% and 5.2% respectively. Colonic administration of butyrate, at physiologically relevant concentrations, dose-dependently decreases visceral sensitivity in healthy volunteers.
结肠微生物对膳食纤维的发酵会产生短链脂肪酸（主要是丙酸、丁酸和乙酸），结肠黏膜会利用这些短链脂肪酸。先前的研究表明，丁酸对氧化应激、炎症和细胞凋亡参数有积极影响。然而，最近对大鼠的研究表明，丁酸会增加内脏敏感性。本研究的目的是确定生理相关浓度的丁酸对健康人类受试者内脏感知的影响。11名健康志愿者参与了这项随机双盲、安慰剂对照的交叉研究。该研究包括三个为期1周的阶段，在此期间，志愿者在睡前每天自行直肠灌注含有100、50 mmol L(-1)丁酸或安慰剂（生理盐水）的灌肠剂。在每个测试阶段开始和结束时进行直肠压力测定，以测量疼痛、便意和不适感。丁酸治疗导致在整个方案压力范围内疼痛、便意和不适感呈剂量依赖性降低。在4 mmHg压力下，50和100 mmol L(-1)的丁酸浓度分别使疼痛评分降低23.9%和42.1%，不适感评分分别降低44.2%和69.0%。在67 mmHg压力下，50和100 mmol L(-1)的丁酸分别使疼痛评分降低23.8%和42%，不适感评分分别降低1.9%和5.2%。生理相关浓度的丁酸经结肠给药可使健康志愿者的内脏敏感性呈剂量依赖性降低。
