Vanella A, Campisi A, Castorina C, Sorrenti V, Attaguile G, Samperi P, Azzia N, Di Giacomo C, Schilirò G
Institute of Biochemistry, University of Catania, Italy.
Pharmacol Res. 1991 Jul;24(1):25-31. doi: 10.1016/1043-6618(91)90061-2.
In the present study we have assayed the effect of divicine in G6PD-deficient red blood cells in the presence of deferoxamine (iron-chelating drug) and NaN3 (inhibitor of catalase). The effect of divicine has been compared to oxidative stress by H2O2; haemolysis is regarded as an index of cellular toxicity. In addition, we have tested antioxidant enzymatic systems. No significant change in antioxidant enzymatic systems was found in RBCs from subjects with G6PD deficiency when compared to the control group, either in oxidative haemolysis by divicine or by H2O2; a significant decrease in oxidative haemolysis by H2O2 was observed in the presence of deferoxamine, whereas no change was found in oxidative haemolysis by divicine. The replacement of incubation medium by homologous plasma or the supplementation with bovine serum albumin resulted in a marked decrease of percentage of haemolysis by divicine.
在本研究中，我们检测了在去铁胺（铁螯合剂）和NaN₃（过氧化氢酶抑制剂）存在的情况下，蚕豆嘧啶对葡萄糖-6-磷酸脱氢酶（G6PD）缺乏的红细胞的影响。已将蚕豆嘧啶的作用与过氧化氢引起的氧化应激进行了比较；溶血被视为细胞毒性的指标。此外，我们还测试了抗氧化酶系统。与对照组相比，G6PD缺乏受试者的红细胞中的抗氧化酶系统在蚕豆嘧啶或过氧化氢引起的氧化溶血中均未发现显著变化；在去铁胺存在的情况下，观察到过氧化氢引起的氧化溶血显著降低，而蚕豆嘧啶引起的氧化溶血未发现变化。用同源血浆替代孵育培养基或补充牛血清白蛋白导致蚕豆嘧啶引起的溶血百分比显著降低。
