Tsamesidis Ioannis, Pantaleo Antonella, Pekou Anna, Gusani Amrita, Iliadis Stavros, Makedou Kali, Manca Alessia, Carruale Antonio, Lymperaki Eugenia, Fozza Claudio
Department of Biomedical Sciences, University of Sassari, Sassari, Italy.
Department of Biochemistry, Aristotle University of Thessaloniki, Thessaloniki, Greece.
Int J Hematol Oncol Stem Cell Res. 2019 Apr 1;13(2):49-57.
Over the last few decades, there has been a dramatic increase in hematological malignancies (HMs) in the population of Sardinia. It is accepted that oxidative stress biomarkers have been demonstrated to be prognostically important in various neoplastic diseases. The aim of this study is to evaluate serum vitamin E, total antioxidant capacity (TAC), Malondialdehyde (MDA) and reactive oxygen species (ROS) levels in 80 Sardinian patients with different HMs [acute myeloid leukemia (AML)(n=20), myelodysplastic syndromes (MDS) (n=20), Hodgkin lymphoma (HL) (n=20) and non-Hodgkin lymphoma (NHL) (n=20)] on the day of their diagnosis. Samples from all participants were obtained after an overnight fast (at least 10 hours). This study was approved and conducted in accordance with Good Clinical Practice guidelines and the Declaration of Helsinki. Patients and controls provided written, informed consent before entering the study. All study participants' medical history and their medication were documented upon enrolling. Lower levels of TAC and Vitamin E were observed in most of the studied groups compared to healthy controls (0.41-0.49 mmol/L vs. 0.56 mmol/L) (19.55-28.55 μmol/L vs. 34.51 μmol/L). Moreover, higher average MDA levels were observed in HL and NHL patients compared to healthy controls (16.6 ng/ml-17.8 ng/ml vs. 7.4 ng/ml). Additionally, the ROS values of all studied groups were found elevated. Serum TAC showed significant negative correlations with MDA values (R= -0.51; P<0.001). Statistical significance was observed in all hematological parameters, producing either positive or negative correlation with at least one OS biomarker. The present data suggest that Sardinian patients with HL and NHL on the day of their diagnosis presented the highest OS in comparison to AML and healthy subjects. Moreover, MDS patients presented high OS status. Likewise, our results also indicated that changes in their hematological indices are eminent of their oxidative and antioxidative status.
在过去几十年中，撒丁岛人群中的血液系统恶性肿瘤（HMs）显著增加。氧化应激生物标志物已被证明在各种肿瘤疾病中具有重要的预后意义。本研究的目的是评估80例不同血液系统恶性肿瘤（急性髓系白血病（AML）（n = 20）、骨髓增生异常综合征（MDS）（n = 20）、霍奇金淋巴瘤（HL）（n = 20）和非霍奇金淋巴瘤（NHL）（n = 20））的撒丁岛患者在诊断当天的血清维生素E、总抗氧化能力（TAC）、丙二醛（MDA）和活性氧（ROS）水平。所有参与者的样本均在禁食过夜（至少10小时）后采集。本研究已获批准，并按照良好临床实践指南和《赫尔辛基宣言》进行。患者和对照在进入研究前提供了书面知情同意书。所有研究参与者的病史和用药情况在入组时均有记录。与健康对照相比（0.41 - 0.49 mmol/L对0.56 mmol/L）（19.55 - 28.55 μmol/L对34.51 μmol/L），在大多数研究组中观察到TAC和维生素E水平较低。此外，与健康对照相比（16.6 ng/ml - 17.8 ng/ml对7.4 ng/ml），HL和NHL患者的平均MDA水平较高。此外，所有研究组的ROS值均升高。血清TAC与MDA值呈显著负相关（R = -0.51；P < 0.001）。在所有血液学参数中均观察到统计学意义，与至少一种OS生物标志物呈正相关或负相关。目前的数据表明，与AML患者和健康受试者相比，撒丁岛HL和NHL患者在诊断当天的OS最高。此外，MDS患者呈现高OS状态。同样，我们的结果还表明，他们血液学指标的变化与其氧化和抗氧化状态密切相关。
