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朊病毒蛋白（PrPSc）的物种和毒株糖基化模式

Species and strain glycosylation patterns of PrPSc.

作者信息

Xanthopoulos Konstantinos, Polymenidou Magdalini, Bellworthy Sue J, Benestad Sylvie L, Sklaviadis Theodoros

机构信息

Laboratory of Pharmacology, Department of Pharmacy, Aristotle University of Thessaloniki, Thessaloniki, Greece.

出版信息

PLoS One. 2009 May 20;4(5):e5633. doi: 10.1371/journal.pone.0005633.

DOI:10.1371/journal.pone.0005633
PMID:19461968
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2680983/
Abstract

BACKGROUND

A key event in transmissible spongiform encephalopathies (TSEs) is the conversion of the soluble, protease-sensitive glycosylated prion protein (PrP(C)) to an abnormally structured, aggregated and partially protease-resistant isoform (PrP(Sc)). Both PrP isoforms bear two potential glycosylation sites and thus in a typical western blot with an anti-PrP antibody three distinct bands appear, corresponding to the di-, mono- or unglycosylated forms of the protein. The relative intensity and electrophoretic mobility of the three bands are characteristic of each TSE strain and have been used to discriminate between them.

METHODOLOGY/PRINCIPAL FINDINGS: In the present study we used lectin-based western blotting to evaluate possible variations in composition within sugar chains carried by PrP(Sc) purified from subjects affected with different TSEs. Our findings indicate that in addition to the already well-documented differences in electrophoretic mobility and amounts of the glycosylated PrP(Sc) forms, TSE strains also vary in the abundance of specific N-linked sugars of the PrP(Sc) protein.

CONCLUSIONS/SIGNIFICANCE: These results imply that PrP glycosylation might fine-tune the conversion of PrP(C) to PrP(Sc) and could play an accessory role in the appearance of some of the characteristic features of TSE strains. The differences in sugar composition could also be used as an additional tool for discrimination between the various TSEs.

摘要

背景

传染性海绵状脑病（TSEs）中的一个关键事件是可溶性、蛋白酶敏感的糖基化朊病毒蛋白（PrP(C)）转变为结构异常、聚集且部分抗蛋白酶的异构体（PrP(Sc)）。两种PrP异构体都有两个潜在的糖基化位点，因此在典型的用抗PrP抗体进行的蛋白质印迹中会出现三条不同的条带，分别对应蛋白质的双糖基化、单糖基化或无糖基化形式。这三条条带的相对强度和电泳迁移率是每种TSE毒株的特征，并已被用于区分它们。

方法/主要发现：在本研究中，我们使用基于凝集素的蛋白质印迹法来评估从患有不同TSEs的受试者中纯化的PrP(Sc)所携带糖链组成的可能变化。我们的发现表明，除了已充分记录的糖基化PrP(Sc)形式的电泳迁移率和含量差异外，TSE毒株在PrP(Sc)蛋白特定N-连接糖的丰度上也有所不同。

结论/意义：这些结果表明，PrP糖基化可能会微调PrP(C)向PrP(Sc)的转变，并可能在TSE毒株某些特征的出现中起辅助作用。糖组成的差异也可作为区分各种TSEs的额外工具。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3de/2680983/808f124ad8a9/pone.0005633.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3de/2680983/cb4cec17a3bf/pone.0005633.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3de/2680983/fb84f87faba4/pone.0005633.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3de/2680983/416270cb4f9d/pone.0005633.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3de/2680983/808f124ad8a9/pone.0005633.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3de/2680983/cb4cec17a3bf/pone.0005633.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3de/2680983/fb84f87faba4/pone.0005633.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3de/2680983/416270cb4f9d/pone.0005633.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3de/2680983/808f124ad8a9/pone.0005633.g004.jpg

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本文引用的文献

1
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2
A systems approach to prion disease.一种针对朊病毒疾病的系统方法。
Mol Syst Biol. 2009;5:252. doi: 10.1038/msb.2009.10. Epub 2009 Mar 24.
3
Prion protein glycosylation is not required for strain-specific neurotropism.朊病毒蛋白糖基化对于毒株特异性嗜神经性并非必需。
朊病毒：超越单一蛋白质
Clin Microbiol Rev. 2016 Jul;29(3):633-58. doi: 10.1128/CMR.00046-15.
4
Assessing proteinase K resistance of fish prion proteins in a scrapie-infected mouse neuroblastoma cell line.在感染羊瘙痒病的小鼠神经母细胞瘤细胞系中评估鱼类朊病毒蛋白的蛋白酶K抗性。
Viruses. 2014 Nov 13;6(11):4398-421. doi: 10.3390/v6114398.
5
Glycoform-selective prion formation in sporadic and familial forms of prion disease.糖型选择性朊病毒形成在散发性和家族性朊病毒病中。
PLoS One. 2013;8(3):e58786. doi: 10.1371/journal.pone.0058786. Epub 2013 Mar 19.
J Virol. 2009 Jun;83(11):5321-8. doi: 10.1128/JVI.02502-08. Epub 2009 Mar 18.
4
Glycosylation-related genes are variably expressed depending on the differentiation state of a bioaminergic neuronal cell line: implication for the cellular prion protein.糖基化相关基因的表达因生物胺能神经元细胞系的分化状态而异：对细胞朊蛋白的影响。
Glycoconj J. 2009 May;26(4):477-93. doi: 10.1007/s10719-008-9198-5. Epub 2008 Oct 21.
5
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6
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