Arsac Jean-Noël, Andreoletti Olivier, Bilheude Jean-Marc, Lacroux Caroline, Benestad Sylvie L, Baron Thierry
Agence Française de Sécurité Sanitaire des Aliments, Lyon, France.
Emerg Infect Dis. 2007 Jan;13(1):58-65. doi: 10.3201/eid1301.060393.
Isolates of atypical scrapie recently identified in sheep and goats in France were compared with Nor98 isolates reported in Norway. Western blot methods for characterization of the protease-resistant prion protein showed that all these isolates shared a unique biochemical signature: 5 groups of bands, including a characteristic band of apparent low molecular weight (11 kDa). This pattern could originate from the presence of 3 different protease cleavage products, including the 11 kDa most likely cleaved at both N- and C-sides of the protein. Genetic data, which strongly suggested the higher susceptibility of AHQ and AF141RQ animals in French cases, resembled earlier data from Nor98 scrapie.
将法国绵羊和山羊中最近鉴定出的非典型羊瘙痒病分离株与挪威报告的Nor98分离株进行了比较。用于表征抗蛋白酶朊病毒蛋白的蛋白质印迹方法表明，所有这些分离株都具有独特的生化特征：5组条带，包括一条明显低分子量（11 kDa）的特征条带。这种模式可能源于3种不同蛋白酶裂解产物的存在，包括最有可能在蛋白质的N端和C端均被裂解的11 kDa产物。遗传数据强烈表明法国病例中AHQ和AF141RQ动物的易感性更高，这与Nor98羊瘙痒病的早期数据相似。
