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M2B细胞的生物学和生物化学特性：经典牛海绵状脑病朊病毒在过表达牛朊病毒蛋白基因的转基因小鼠中得以保留。

Biological and biochemical characterization of M2B cells: Classical BSE prion is conserved in transgenic mice overexpressing bovine prion protein gene.

作者信息

Suh Tae-Young, Roh In Soon, Kim Hyo-Jin, Griffiths Peter C, Park Kyung Je, Park Hoo Chang, Hope James, Kang Hae Eun, Kim Dae-Yong, Sohn Hyun Joo

机构信息

a Animal and Plant Quarantine Agency, Ministry of Agriculture, Food and Rural Affairs , Gimcheon, Gyeongsangbuk-do , Republic of Korea.

b Animal and Plant Health Agency (APHA) , Weybridge, New Haw, Addlestone, Surrey , UK.

出版信息

Prion. 2017 Nov 2;11(6):405-414. doi: 10.1080/19336896.2017.1331809. Epub 2017 Nov 3.

DOI:10.1080/19336896.2017.1331809
PMID:29098930
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5786353/
Abstract

M2B cells with persistent classical bovine spongiform encephalopathy (C-BSE) have been established previously. In this study, we performed strain characterization of the M2B cell line in bovine PrP overexpressing mice (Tg 1896). Mice intracranially inoculated with M2B cells and C-BSE survived for 451 ± 7 and 465 ± 31 d post inoculation, respectively. Although biochemical properties, including deglycosylation and conformational stability, differed between M2B cells and C-BSE, inoculation with M2B cell lysate and C-BSE resulted in comparable phenotypes. Comparable vacuolation scores and PrP depositions were observed in the brain of Tg 1896 inoculated with both M2B cell lysate and C-BSE. Our results show that biochemical and biological characteristics of M2B cells and C-BSE are classifiable in the same strain.

摘要

此前已建立了携带持续性经典牛海绵状脑病（C-BSE）的M2B细胞。在本研究中，我们对过表达牛朊蛋白的小鼠（Tg 1896）中的M2B细胞系进行了毒株特性分析。经颅内接种M2B细胞和C-BSE的小鼠，接种后分别存活了451±7天和465±31天。尽管M2B细胞和C-BSE在包括去糖基化和构象稳定性等生化特性方面存在差异，但接种M2B细胞裂解物和C-BSE产生了相似的表型。在接种了M2B细胞裂解物和C-BSE的Tg 1896小鼠脑中观察到了相似的空泡化评分和朊蛋白沉积。我们的结果表明，M2B细胞和C-BSE的生化及生物学特性可归为同一毒株。

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本文引用的文献

1
Generation of a persistently infected MDBK cell line with natural bovine spongiform encephalopathy (BSE).利用自然发生的牛海绵状脑病（BSE）生成持续感染的MDBK细胞系。
PLoS One. 2015 Feb 3;10(2):e0115939. doi: 10.1371/journal.pone.0115939. eCollection 2015.
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A bovine cell line that can be infected by natural sheep scrapie prions.一种可被天然绵羊瘙痒病朊病毒感染的牛细胞系。
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α-Cleavage of cellular prion protein.α-切割细胞朊病毒蛋白。
Prion. 2012 Nov-Dec;6(5):453-60. doi: 10.4161/pri.22511. Epub 2012 Oct 10.
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Species and strain glycosylation patterns of PrPSc.朊病毒蛋白（PrPSc）的物种和毒株糖基化模式
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