Koen Ia M, Perevoshchikova K A, Zbarskii I B
Biokhimiia. 1976 Jul;41(6):982-8.
A comparative study of glucose-6-phosphatase, alcaline RNase, ATPase, inosine diphosphatase and 5'-nucleotidase activities in isolated rat liver and hepatoma-27 nuclei and nuclear envelopes was performed. The tumor nuclear membranes were shown to be free from G-6-Pase activity in contrast to the liver nuclear membranes. The nuclear RNase activity was strongly inhibited in the hepatoma and could be unmasked in the presence of 3-10(-4) M pCMB. Hepatoma nuclear and nuclear envelopes ATP-ase activity was found to be moderately decreased as compared to those of the normal tissue. The values of inosine diphosphatase activity in hepatoma were similar to those in liver. The role of the nuclear envelope in nuclear-cytoplasmic interactions as well as nuclear location of G-6-Pase are discussed.
对分离的大鼠肝脏、肝癌-27细胞核及核膜中的葡萄糖-6-磷酸酶、碱性核糖核酸酶、三磷酸腺苷酶、肌苷二磷酸酶和5'-核苷酸酶活性进行了比较研究。结果显示，与肝细胞核膜不同，肿瘤核膜无葡萄糖-6-磷酸酶活性。肝癌细胞核中的核糖核酸酶活性受到强烈抑制，在存在3×10⁻⁴M对氯汞苯甲酸时可被揭示出来。与正常组织相比，发现肝癌细胞核及核膜的三磷酸腺苷酶活性中度降低。肝癌中肌苷二磷酸酶活性的值与肝脏中的相似。讨论了核膜在核质相互作用中的作用以及葡萄糖-6-磷酸酶的核定位。
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