Peskin A V, Koen Y M, Zbarsky I B
Mol Biol Rep. 1981 May 22;7(1-3):25-30. doi: 10.1007/BF00778729.
Messenger RNA is released preferentially from isolated rat liver nuclei in the presence of the ATP-generating system and cytosol. The release is suppressed by spermidine, while cytoplasmic RNase inhibitor was ineffective and PCMB like some other thiol-blocking agents inhibitory. Cytoplasmic SOD added to the system strongly suppressed RNA release. A similar effect could be obtained by anaerobiosis due to addition of SMP. In both cases the inhibition is reversed by cyanide. In contrast to normal liver where the generation of superoxide radicals takes place almost exclusively in microsomes and is coupled with the oxidation of NADPH, in mouse ascites hepatoma 22a the generation of superoxide radicals occurs mainly in the nuclear envelope and is coupled wih the oxidation of both NADPH and NADH and inhibited by cyanide.
在存在ATP生成系统和胞质溶胶的情况下，信使核糖核酸优先从分离的大鼠肝细胞核中释放出来。亚精胺可抑制这种释放，而细胞质核糖核酸酶抑制剂无效，对氯汞苯甲酸和其他一些巯基阻断剂一样具有抑制作用。向系统中添加细胞质超氧化物歧化酶可强烈抑制核糖核酸的释放。由于添加了琥珀酸甲酯而导致的厌氧状态也可产生类似效果。在这两种情况下，氰化物均可逆转抑制作用。与正常肝脏中超氧自由基几乎只在微粒体中产生并与NADPH氧化偶联不同，在小鼠腹水肝癌22a中，超氧自由基主要在核膜中产生，并与NADPH和NADH的氧化偶联，且受氰化物抑制。
