Yang Guangming, Chang Yanbing, Yang Hua, Tan Lin, Wu Zaisheng, Lu Xuxiao, Yang Yunhui
College of Chemistry and Chemical Engineering, Yunnan Normal University, Kunming 650092, PR China.
Anal Chim Acta. 2009 Jun 30;644(1-2):72-7. doi: 10.1016/j.aca.2009.04.021. Epub 2009 Apr 23.
A novel amperometric immunosensor for determination of human serum chorionic gonadotrophin (hCG) was constructed by immobilizing hCG antibody with nano-gold and chitosan (CHIT) hybrid film electrochemical co-deposited on a glassy carbon electrode. The fabricated procedures and electrochemical behaviors of proteins on such an interface were characterized with electrochemical impedance, cyclic voltammetry and chronoamperometry. The effect of incubation time on the electrochemical behavior of immunosensor was studied and other experimental conditions such as mass ratio of CHIT and HAuCl4, depositing time of hybrid film, the pH, and temperature were studied too. The current response of the immunosensor is in linear relationship with concentration of hCG. The resulting immunosensor offers an excellent amperometric response for hCG ranging from 0.20 to 100 mIU mL(-1) with a detection limit of 0.1 mIU mL(-1). A satisfactory determination of hCG in serum samples can be obtained by this immunosensor.
通过将人绒毛膜促性腺激素（hCG）抗体固定在纳米金和壳聚糖（CHIT）混合膜上，该混合膜通过电化学共沉积在玻碳电极上，构建了一种用于测定人血清绒毛膜促性腺激素（hCG）的新型安培免疫传感器。用电化学阻抗、循环伏安法和计时电流法对蛋白质在该界面上的制备过程和电化学行为进行了表征。研究了孵育时间对免疫传感器电化学行为的影响，还研究了其他实验条件，如CHIT与HAuCl4的质量比、混合膜的沉积时间、pH值和温度。免疫传感器的电流响应与hCG浓度呈线性关系。所得免疫传感器对hCG在0.20至100 mIU mL(-1)范围内具有出色的安培响应，检测限为0.1 mIU mL(-1)。该免疫传感器可对血清样品中的hCG进行令人满意的测定。
