Department of Chemistry, Zhejiang University, Hangzhou 310027, PR China.
Talanta. 2011 Sep 30;85(4):1980-5. doi: 10.1016/j.talanta.2011.07.012. Epub 2011 Jul 18.
A simple and controllable one-step electrodeposition method for the preparation of a chitosan-carbon nanotubes-gold nanoparticles (CS-CNTs-GNPs) nanocomposite film was used to fabricate an immunosensor for detection of carcinoembryonic antigen (CEA). The porous three-dimensional CS-CNTs-GNPs nanocomposite film, which offered a large specific surface area for immobilization of antibodies, exhibited improved conductivity, high stability and good biocompatibility. The morphology of the formed nanocomposite film was investigated by scanning electron microscopy (SEM), and the electrochemical behaviors of the immunosensor were characterized by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). Under the optimal conditions, the proposed immunosensor could detect CEA in two linear ranges from 0.1 to 2.0 ng mL(-1) and from 2.0 to 200.0 ng mL(-1), with a detection limit of 0.04 ng mL(-1). The immunosensor based on CS-CNTs-GNPs nanocomposite film as the antibody immobilization matrix could exhibit good sensitivity, stability, and reproducibility for the determination of CEA.
一种简单可控的一步电沉积法用于制备壳聚糖-碳纳米管-金纳米粒子（CS-CNTs-GNPs）纳米复合膜，用于制备用于检测癌胚抗原（CEA）的免疫传感器。多孔三维 CS-CNTs-GNPs 纳米复合膜为抗体的固定提供了大的比表面积，表现出改善的导电性、高稳定性和良好的生物相容性。通过扫描电子显微镜（SEM）研究了形成的纳米复合膜的形态，通过电化学阻抗谱（EIS）和循环伏安法（CV）表征了免疫传感器的电化学行为。在最佳条件下，所提出的免疫传感器可以在 0.1 至 2.0 ng mL(-1)和 2.0 至 200.0 ng mL(-1)的两个线性范围内检测 CEA，检测限为 0.04 ng mL(-1)。基于 CS-CNTs-GNPs 纳米复合膜作为抗体固定基质的免疫传感器可以表现出良好的灵敏度、稳定性和重现性，用于测定 CEA。
