Ye Yanrui, Zhu Yi, Pan Li, Li Lili, Wang Xiaoning, Lin Ying
School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, PR China.
Biochem Biophys Res Commun. 2009 Jul 31;385(3):357-62. doi: 10.1016/j.bbrc.2009.05.071. Epub 2009 May 20.
Extensive alteration of gene expression and metabolic remodeling enable the budding yeast Saccharomyces cerevisiae to ensure cellular homeostasis and adaptation to heat shock. The response logic of the cells to heat shock is still not entirely clear. In this study, we combined the expression profiles with metabolic pathways to investigate the logical relations between heat shock response metabolic pathways. The results showed that the heat-stressed S. cerevisiae cell accumulated trehalose and glycogen, which protect cellular proteins against denaturation, and modulate its phospholipid structure to sustain stability of the cell wall. The TCA cycle was enhanced, and the heat shock-induced turnover of amino acids and nucleotides served to meet the extra energy requirement due to heat-induced protein metabolism and modification. The enhanced respiration led to oxidative stress, and subsequently induced the aldehyde detoxification system. These results indicated that new insight into the response logic of S. cerevisiae to heat shock can be gained by integrating expression profiles and the logical relations between heat shock response metabolic pathways.
基因表达的广泛改变和代谢重塑使酿酒酵母能够确保细胞内稳态并适应热休克。细胞对热休克的反应逻辑仍不完全清楚。在本研究中，我们将表达谱与代谢途径相结合，以研究热休克反应代谢途径之间的逻辑关系。结果表明，热应激的酿酒酵母细胞积累海藻糖和糖原，保护细胞蛋白质免受热变性，并调节其磷脂结构以维持细胞壁的稳定性。三羧酸循环增强，热休克诱导的氨基酸和核苷酸周转用于满足热诱导的蛋白质代谢和修饰所产生的额外能量需求。增强的呼吸作用导致氧化应激，随后诱导醛解毒系统。这些结果表明，通过整合表达谱和热休克反应代谢途径之间的逻辑关系，可以获得对酿酒酵母热休克反应逻辑的新见解。
