Centre for Soil and Environmental Science, Department of Agronomy, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, Sl-1000 Ljubljana, Slovenia.
Environ Pollut. 2009 Oct;157(10):2822-9. doi: 10.1016/j.envpol.2009.04.023. Epub 2009 May 22.
Leaching using EDTA applied to a Pb, Zn and Cd polluted soil significantly reduced soil metal concentrations and the pool of metals in labile soil fractions. Metal mobility (Toxicity Characteristic Leaching Procedure), phytoavailability (diethylenetriaminepentaacetic acid extraction) and human oral-bioavailability (Physiologically Based Extraction Test) were reduced by 85-92%, 68-91% and 88-95%, respectively. The metal accumulation capacity of the terrestrial isopod Porcellio scaber (Crustacea) was used as in vivo assay of metal bioavailability, before and after soil remediation. After feeding on metal contaminated soil for two weeks, P. scaber accumulated Pb, Zn and Cd in a concentration dependent manner. The amounts of accumulated metals were, however, higher than expected on the basis of extraction (in vitro) tests. The combined results of chemical extractions and the in vivo test with P. scaber provide a more relevant picture of the availability stripping of metals after soil remediation.
螯合剂 EDTA 浸提应用于 Pb、Zn 和 Cd 污染土壤，可显著降低土壤金属浓度和可利用土壤部分中的金属库。金属迁移性（毒性特征浸出程序）、植物可利用性（二乙三胺五乙酸提取）和人体口服生物利用度（基于生理的提取测试）分别降低了 85-92%、68-91%和 88-95%。在土壤修复前后，使用陆生等足目动物 P. scaber（甲壳纲）的金属积累能力作为金属生物利用度的体内测定。在食用受金属污染的土壤两周后，P. scaber 以浓度依赖的方式积累 Pb、Zn 和 Cd。然而，积累的金属量高于基于提取（体外）测试的预期。化学提取和 P. scaber 体内测试的综合结果提供了更相关的图片，说明土壤修复后金属的可用性降低。
