Surzycki Raymond, Greenham Katie, Kitayama Kaoru, Dibal Flora, Wagner Richard, Rochaix Jean-David, Ajam Tarek, Surzycki Stefan
PhycoBiologics Inc, Indianapolis IN, Bloomington, IN 47401, USA.
Biologicals. 2009 Jun;37(3):133-8. doi: 10.1016/j.biologicals.2009.02.005.
PhycoBiologics is developing an oral vaccine delivery system using vaccines expressed in the chloroplast of microalgae. Despite many advances in plastid transformation technology, levels of expression remain inconsistent. We have concluded that the main factors affecting the level of recombinant protein expression in the chloroplast of Chlamydomonas are: codon optimization, protease activity, protein toxicity and transformation-associated genotypic modification.
藻生物制剂公司正在研发一种口服疫苗递送系统，该系统使用在微藻叶绿体中表达的疫苗。尽管质体转化技术取得了许多进展，但表达水平仍然不一致。我们得出结论，影响衣藻叶绿体中重组蛋白表达水平的主要因素有：密码子优化、蛋白酶活性、蛋白质毒性和转化相关的基因型修饰。
