Purwosunu Yuditiya, Sekizawa Akihiko, Yoshimura Shiho, Farina Antonio, Wibowo Noroyono, Nakamura Masamitsu, Shimizu Hanako, Okai Takashi
Department of Obstetrics and Gynecology, University of Indonesia, Cipto Mangunkusumo National Hospital, Jakarta, Indonesia.
Reprod Sci. 2009 Sep;16(9):857-64. doi: 10.1177/1933719109336622. Epub 2009 May 27.
The purpose of this study is to assess the changes in the expression of angiogenesis-related genes in the cellular component of the blood from preeclamptic patients. Blood samples were obtained from the preeclampsia (PE) and control participants. Cellular RNA was analyzed by reverse transcription polymerase chain reaction (PCR) to identify any angiogenesis-related genes and thereby assess the mRNA expression among women with PE and controls during weeks 35 to 41 of gestation. Significant differences were observed between PE and controls in all of the angiogenesis-related genes examined. In PE, for the increased expression of transforming growth factor-beta1 (TGF-beta1), endoglin and fms-like tyrosine kinase-1 (Flt-1); and the reduced expression of vascular endothelial growth factor (VEGF), placental growth factor (PlGF). fms-Like tyrosine kinase-1 and endoglin significantly correlated with the systolic pressure, while VEGF, Flt-1, and endoglin all correlated with proteinuria. An altered expression of angiogenesis-related genes was demonstrated in the cellular component of blood from preeclamptic patients. These findings indicate that this approach may offer an alternative way for evaluating the pathogenesis of PE.
本研究的目的是评估子痫前期患者血液细胞成分中血管生成相关基因表达的变化。从子痫前期（PE）患者和对照参与者中采集血样。通过逆转录聚合酶链反应（PCR）分析细胞RNA，以鉴定任何血管生成相关基因，从而评估妊娠第35至41周期间PE患者和对照者之间的mRNA表达。在所检测的所有血管生成相关基因中，PE患者与对照者之间均观察到显著差异。在PE患者中，转化生长因子-β1（TGF-β1）、内皮糖蛋白和fms样酪氨酸激酶-1（Flt-1）表达增加；而血管内皮生长因子（VEGF）、胎盘生长因子（PlGF）表达降低。fms样酪氨酸激酶-1和内皮糖蛋白与收缩压显著相关，而VEGF、Flt-1和内皮糖蛋白均与蛋白尿相关。子痫前期患者血液细胞成分中血管生成相关基因表达发生改变。这些发现表明，这种方法可能为评估PE的发病机制提供一种替代途径。
