Nikuei Pooneh, Rajaei Minoo, Malekzadeh Kianoosh, Nejatizadeh Azim, Mohseni Fatemeh, Poordarvishi Fatemeh, Ghashghaeezadeh Nasrin, Mohtarami Mehrdad
Molecular Medicine Research Center, Hormozgan Health Institute, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
Fertility and Infertility Research Center, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
Int J Reprod Biomed. 2017 Mar;15(3):169-174.
Preeclampsia (PE) is a serious complication of pregnancy with hallmarks of incomplete placentation, placental ischemia and endothelial dysfunction. Imbalance between vascular endothelial growth factor (VEGF), placenta growth factor (PlGF) and their receptors play important role in pathophysiology of PE.
This study was aimed to asses PlGF mRNA expression in placenta of women affected with PE.
In this cross-sectional study, expression of PlGF mRNA was evaluated in 26 mild PE cases, 15 severe preeclamptic women and 20 normotensive controls. Patients were sub-classified as early onset PE (9) and late onset (32). After RNA extraction, PlGF expression was quantified with qRT-PCR.
The results of PlGF mRNA expression between mild-severe, and early-late onset PE patients showed no statistically significant difference compared with the control group (p=0.661, p=0.205 respectively).
Despite we found no distinct differential expression of PlGF mRNA in placental tissue of PE patients compared with control women, but according to decreased level of this angiogenic factor in PE even before clinical onset of the disease, determining molecular mechanisms related to reduced secretion of PlGF into the maternal circulation may be useful for future therapeutics.
子痫前期（PE）是一种严重的妊娠并发症，其特征为胎盘形成不全、胎盘缺血和内皮功能障碍。血管内皮生长因子（VEGF）、胎盘生长因子（PlGF）及其受体之间的失衡在PE的病理生理学中起重要作用。
本研究旨在评估PE患者胎盘组织中PlGF mRNA的表达情况。
在这项横断面研究中，对26例轻度PE患者、15例重度子痫前期患者和20例血压正常的对照者的胎盘组织中PlGF mRNA的表达进行了评估。患者被分为早发型PE（9例）和晚发型（32例）。提取RNA后，采用qRT-PCR对PlGF表达进行定量分析。
轻度-重度、早发型-晚发型PE患者之间PlGF mRNA表达结果与对照组相比，差异均无统计学意义（分别为p = 0.661，p = 0.205）。
尽管我们发现PE患者胎盘组织中PlGF mRNA的表达与对照女性相比无明显差异，但鉴于在疾病临床发作前PE患者体内这种血管生成因子水平就已降低，确定与PlGF分泌减少至母体循环相关的分子机制可能对未来治疗有帮助。
