Bourtourault M, Shacoori V, Guerin J, Saiag B, Rault B
Laboratoire de physiologie pharmaceutique, Faculté de pharmacie, Rennes, France.
Res Commun Chem Pathol Pharmacol. 1991 Jun;72(3):273-84.
Female rats were immunized with mixed 17 beta-estradiol-6-carboxymethyloxime-bovine serum albumin and testosterone-3-carboxymethyloxime-bovine serum albumin. All the animals produced antibodies against the 2 haptens and were better immunized against 17 beta-estradiol than against testosterone. Nevertheless the disappearance of cyclic ovarian function was only obtained in animals highly immunized against the two antigens. The 17 beta-estradiol serum concentration increased with the level of immunization whereas the progesterone concentration fell. Persistent vaginal cornification was observed and ovarian lesions (large fluid filled cysts, hemorrhagic follicles and absence of corpora lutea) coincided with increased serum levels of luteinizing hormone and prolactin.
用混合的17β-雌二醇-6-羧甲基肟-牛血清白蛋白和睾酮-3-羧甲基肟-牛血清白蛋白对雌性大鼠进行免疫。所有动物均产生了针对这两种半抗原的抗体，并且对17β-雌二醇的免疫效果比对睾酮的更好。然而，只有在对两种抗原进行高度免疫的动物中才出现周期性卵巢功能的消失。17β-雌二醇血清浓度随免疫水平升高而增加，而孕酮浓度下降。观察到持续性阴道角化，并且卵巢病变（充满液体的大囊肿、出血性卵泡和黄体缺失）与促黄体生成素和催乳素的血清水平升高同时出现。
