用于高通量应用的活细胞光学传感。

Live cell optical sensing for high throughput applications.

机构信息

Biochemical Department, Science and Technology Division, Corning Incorporated, Corning, New York, 14831, USA,

出版信息

Adv Biochem Eng Biotechnol. 2010;118:153-63. doi: 10.1007/10_2009_4.

DOI:10.1007/10_2009_4

PMID:19475370

Abstract

Live cell optical sensing employs label-free optical biosensors to non-invasively measure stimulus-induced dynamic mass redistribution (DMR) in live cells within the sensing volume of the biosensor. The resultant DMR signal is an integrated cellular response, and reflects cell signaling mediated through the cellular target(s) with which the stimulus intervenes. This article describes the uses of live cell optical sensing for probing cell biology and ligand pharmacology, with an emphasis of resonant waveguide grating biosensor cellular assays for high throughput applications.

摘要

活细胞光学传感采用无标记的光学生物传感器，非侵入式地测量生物传感器感应体积内活细胞中因刺激引起的动态质量重分布（DMR）。所得的 DMR 信号是细胞的综合反应，反映了通过细胞靶标（刺激物与这些靶标相互作用）介导的细胞信号转导。本文描述了活细胞光学传感在探测细胞生物学和配体药理学中的应用，重点介绍了用于高通量应用的共振波导光栅生物传感器细胞分析。