Luo Wen, Zhou Xiaodong, Yu Ming, He Guangbin, Zheng Xiaoying, Li Qiuyang, Liu Qing, Han Zenghui, Zhang Jun, Qian Yunqiu
Department of Ultrasound, Xijing Hospital, Fourth Military Medical University, Shaanxi, China.
Ann Surg Oncol. 2009 Aug;16(8):2359-68. doi: 10.1245/s10434-009-0419-3. Epub 2009 May 28.
We investigated sequential effects of HIFU ablation combined with contrast agent SonoVue by using histopathology examination, immunohistochemistry, and enzyme histochemistry.
Forty rabbits with VX2 liver tumors were subjected to HIFU ablation. Before ablation, a bolus injection of 0.2 mL SonoVue was administrated in group II (n = 20), and normal saline solution was injected in group I (n = 20). On day 0, 3, 7, and 14 after ablation, 5 animals in each group were sacrificed. The tissue in ablated zone, transient zone (within 3 mm around ablated area), and surrounding zone (beyond 3 mm around ablated area) were collected. Coagulated volume measurement, hematoxylin-eosin staining, immunohistochemistry of Ki 67, Bcl-2, CD54, and MMP-2 to determine cell proliferation and tissue repair, and nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) and succinic dehydrogenase (SDH) staining to evaluate tissue viability were performed.
The coagulated volume in group II at each time point was larger than that in group I (P < .05). After day 3, hematoxylin-eosin staining demonstrated necrosis in ablated zones and increasing surrounding fibra bands in group I and group II, while increasing expression of Ki 67, Bcl-2, CD54, and MMP-2 in transient zones was detected using immunohistochemistry in both groups (P > .05). NADPH-d and SDH staining showed dramatic decrease of enzyme activities in ablated zones immediately after ablation, while residual viable tissues in ablated zones of group II were less than those of group I (P < .05).
Contrast agent SonoVue enables improvement of HIFU ablation on rabbit VX2 liver tumors.
我们通过组织病理学检查、免疫组织化学和酶组织化学研究了高强度聚焦超声（HIFU）消融联合造影剂声诺维的序贯效应。
40只患有VX2肝肿瘤的兔子接受HIFU消融。消融前，第二组（n = 20）静脉推注0.2 mL声诺维，第一组（n = 20）注射生理盐水。在消融后第0、3、7和14天，每组处死5只动物。收集消融区、过渡区（消融区域周围3 mm内）和周边区（消融区域周围3 mm外）的组织。进行凝固体积测量、苏木精-伊红染色、Ki 67、Bcl-2、CD54和MMP-2的免疫组织化学以确定细胞增殖和组织修复，以及烟酰胺腺嘌呤二核苷酸磷酸黄递酶（NADPH-d）和琥珀酸脱氢酶（SDH）染色以评估组织活力。
第二组在每个时间点的凝固体积均大于第一组（P <.05）。消融后第3天，苏木精-伊红染色显示两组消融区均有坏死，周边纤维带增多，而两组过渡区Ki 67、Bcl-2、CD54和MMP-2的免疫组织化学表达均增加（P >.05）。NADPH-d和SDH染色显示消融后即刻消融区酶活性显著降低，而第二组消融区的残余存活组织少于第一组（P <.05）。
造影剂声诺维可改善HIFU对兔VX2肝肿瘤的消融效果。
