Kamal Md Mostofa, Alam Md Emtiaj, Das Sunny Kumar, Yeasmin Most Shorifa, Ahmed Soshe, Rahman Mst Afroza, Das Dipak Kumar, Gofur Md Royhan, Masum Md Abdul
Department of Veterinary and Animal Sciences, Faculty of Veterinary and Animal Sciences, Rajshahi University, Rajshahi, Bangladesh.
Department of Pharmacy, Faculty of Sciences and Engineering, East West University, Dhaka, Bangladesh.
J Adv Vet Anim Res. 2023 Jun 30;10(2):169-177. doi: 10.5455/javar.2023.j666. eCollection 2023 Jun.
This study aimed to examine the impacts of the wide range of concentrations of glucose and trehalose on the tris-citric acid-egg yolk-fructose (TCEF) extenders for cryopreservation of goat semen.
The sperm sample was pooled, washed, and diluted in control (TCEF without glucose and trehalose), TCEF + glucose (75, 150, 450, and 900 mm), and TCEF + trehalose (75, 150, 450, and 900 mm). After equilibrations, the semen straws were frozen under LN2 in the LN2 tank. After LN2 storage, the straws were thawed at 37°C for 30 seconds. The sperm parameters of all study groups were checked after equilibration and freezing.
After equilibration, the progressive motility (PM), total motility (TM), and viability of sperm in G-75, G-150, G-450, T-75, T-150, and T-450 were not significantly different ( < 0.05) from those in control. After cryopreservation and thawing, the PM, TM, and plasma membrane integrity (PMI) of T-150 were significantly higher ( < 0.05) than in control, G-75, G-900, T-75, and T-900. The viability of sperm in T-150 was substantially higher ( < 0.05) than in the control, whereas there was no significant difference among the control, G-75, G-900, T-75, and T-900. However, the acrosome integrity (AI) of sperm in G-900 was significantly decreased ( < 0.05) compared to the control, G-75, G-150, G-450, T-75, T-150, and T-450.
According to the findings, the supplementation of 150 mm trehalose in the TCEF diluent was more efficient for sperm cryopreservation in the buck as reflected by PM, TM, viability, PMI, and AI.
本研究旨在考察不同浓度的葡萄糖和海藻糖对用于山羊精液冷冻保存的三柠檬酸 - 蛋黄 - 果糖（TCEF）稀释液的影响。
收集精子样本，洗涤后分别用对照（不含葡萄糖和海藻糖的TCEF）、TCEF + 葡萄糖（75、150、450和900 mM）以及TCEF + 海藻糖（75、150、450和900 mM）进行稀释。平衡后，将精液细管在液氮罐中于液氮下冷冻。液氮保存后，细管在37°C解冻30秒。在平衡和冷冻后检查所有研究组的精子参数。
平衡后，G - 75、G - 150、G - 450、T - 75、T - 150和T - 450组精子的前向运动率（PM）、总运动率（TM）和活力与对照组相比无显著差异（P < 0.05）。冷冻保存和解冻后，T - 150组的PM、TM和质膜完整性（PMI）显著高于对照组、G - 75、G - 900、T - 75和T - 900组（P < 0.05）。T - 150组精子的活力显著高于对照组（P < 0.05），而对照组、G - 75、G - 900、T - 75和T - 900组之间无显著差异。然而，与对照组、G - 75.G - 150、G - 450、T - 75、T - 150和T - 450组相比，G - 900组精子的顶体完整性（AI）显著降低（P < 0.05）。
根据研究结果，在TCEF稀释液中添加150 mM海藻糖对公羊精子冷冻保存更有效，这在PM、TM、活力、PMI和AI方面均有体现。
