Huestis W H
J Biol Chem. 1977 Oct 10;252(19):6764-8.
Treatment of human erythrocytes with phospholipid vesicles induces a selective membrane permeability defect which leads to osmotic lysis. The defective cells exhibit a massive sodium ion leak while maintaining normal impermeability to other cations, anions, and neutral small molecules. The sodium ion influx and resulting hemolysis may be inhibited by increased pH, by tetrodotoxin, and by reintroduction of vesicle-extracted proteins into the cell. These characteristics suggest that phospholipid vesicle treatment destroys the cell by disrupting a membrane protein system involved in regulation of cation permeability.
用磷脂囊泡处理人红细胞会诱导选择性膜通透性缺陷，进而导致渗透性溶血。有缺陷的细胞表现出大量钠离子泄漏，同时对其他阳离子、阴离子和中性小分子保持正常的不透性。增加pH值、使用河豚毒素以及将从囊泡中提取的蛋白质重新引入细胞中，可抑制钠离子内流和由此导致的溶血。这些特征表明，磷脂囊泡处理通过破坏参与阳离子通透性调节的膜蛋白系统来破坏细胞。
