Saini Sarbjit S, Paterniti Miya, Vasagar Kavitha, Gibbons Scott P, Sterba Patricia M, Vonakis Becky M
Department of Medicine, Division of Allergy and Clinical Immunology, The Johns Hopkins University School of Medicine, Baltimore MD 21224, USA.
Clin Immunol. 2009 Sep;132(3):342-8. doi: 10.1016/j.clim.2009.05.003. Epub 2009 May 27.
Recently, signaling changes in the FcvarepsilonRI pathway involving inositol lipid phosphatases have been identified in the basophils of chronic idiopathic urticaria (CIU) subjects. Based on the profile of basophil FcvarepsilonRI-mediated histamine degranulation, we have segregated CIU subjects into two groups, CIU Responder (CIU R) or CIU Nonresponder (CIU NR). In the present study, we compared expression of SHIP-1, SHIP-2, and Syk protein to histamine release (HR) from mast cells (MC) cultured from the peripheral blood of CIU R, CIU NR, and normal subjects. The MC of CIU R donors contained significantly increased Syk and decreased SHIP-2 as compared to CIU NR (Syk: p=0.038, SHIP-2: p=0.038) and normals (Syk: p=0.042, SHIP-2: p=0.027). Spontaneous HR from CIU donors was increased two-fold compared to normals (p=0.04). In summary, our results suggest a possible predilection for urticarial MC to spontaneously degranulate upon IgE sensitization contributing to the increased pruritus associated with CIU.
最近，在慢性特发性荨麻疹（CIU）患者的嗜碱性粒细胞中发现了涉及肌醇脂质磷酸酶的FcεRI途径的信号变化。基于嗜碱性粒细胞FcεRI介导的组胺脱颗粒情况，我们将CIU患者分为两组，即CIU反应者（CIU R）或CIU无反应者（CIU NR）。在本研究中，我们比较了CIU R、CIU NR和正常受试者外周血培养的肥大细胞（MC）中SHIP-1、SHIP-2和Syk蛋白的表达与组胺释放（HR）的关系。与CIU NR（Syk：p = 0.038，SHIP-2：p = 0.038）和正常受试者（Syk：p = 0.042，SHIP-2：p = 0.027）相比，CIU R供体的MC中Syk显著增加，SHIP-2减少。CIU供体的自发性HR比正常受试者增加了两倍（p = 0.04）。总之，我们的结果表明，荨麻疹MC可能倾向于在IgE致敏后自发脱颗粒，这导致了与CIU相关的瘙痒加剧。
