Godula Kamil, Rabuka David, Nam Ki Tae, Bertozzi Carolyn R
Department of Chemistry, University of California and The Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
Angew Chem Int Ed Engl. 2009;48(27):4973-6. doi: 10.1002/anie.200805756.
Click to view: Glycopolymers can be used to display glycans on microarrays in native-like architectures. The structurally uniform alkyne-terminated mucin mimetic glycopolymers (see picture; TR = fluorophore) were printed on azide-functionalized chips by microcontact printing in the presence of a copper catalyst. The surface-bound glycopolymers bind lectins in a ligand-specific manner.
糖聚合物可用于在类似天然结构的微阵列上展示聚糖。结构均匀的炔基封端的粘蛋白模拟糖聚合物(见图;TR = 荧光团)在铜催化剂存在下通过微接触印刷法印在叠氮化物功能化芯片上。表面结合的糖聚合物以配体特异性方式结合凝集素。