一种对可卡因敏感的大鼠多巴胺转运体的克隆与表达

Cloning and expression of a cocaine-sensitive rat dopamine transporter.

作者信息

Kilty J E, Lorang D, Amara S G

机构信息

Yale University, New Haven, CT 06510.

出版信息

Science. 1991 Oct 25;254(5031):578-9. doi: 10.1126/science.1948035.

DOI:10.1126/science.1948035

PMID:1948035

Abstract

The action of dopamine and other monoamine neurotransmitters at synapses is terminated predominantly by high-affinity reuptake into presynaptic terminals by specific sodium-dependent neurotransmitter transport proteins. A complementary DNA encoding a rat dopamine transporter has been isolated that exhibits high sequence similarity with the previously cloned norepinephrine and gamma-aminobutyric acid transporters. Transient expression of the complementary DNA in HeLa cells confirms the cocaine sensitivity of this transporter.

摘要

多巴胺和其他单胺类神经递质在突触处的作用主要通过特定的钠依赖性神经递质转运蛋白以高亲和力重新摄取到突触前终末而终止。已分离出一种编码大鼠多巴胺转运体的互补DNA，它与先前克隆的去甲肾上腺素和γ-氨基丁酸转运体具有高度的序列相似性。该互补DNA在HeLa细胞中的瞬时表达证实了这种转运体对可卡因的敏感性。