可卡因敏感的多巴胺转运体互补DNA的克隆与表达
Cloning and expression of a cocaine-sensitive dopamine transporter complementary DNA.
作者信息
Shimada S, Kitayama S, Lin C L, Patel A, Nanthakumar E, Gregor P, Kuhar M, Uhl G
机构信息
Laboratory of Molecular Neurobiology, National Institute on Drug Abuse, Baltimore, MD 21224.
出版信息
Science. 1991 Oct 25;254(5031):576-8. doi: 10.1126/science.1948034.
A rat dopamine (DA) transporter complementary DNA has been isolated with combined complementary DNA homology and expression approaches. The DA transporter is a 619-amino acid protein with 12 hydrophobic putative membrane-spanning domains and homology to the norepinephrine and gamma-aminobutyric acid transporters. The expressed complementary DNA confers transport of [3H]DA in Xenopus oocytes and in COS cells. Binding of the cocaine analog [3H]CFT ([3H]2 beta-carbomethoxy-3 beta-(4-fluorophenyl)tropane) to transfected COS cell membranes yields a pharmacological profile similar to that in striatal membranes.
通过互补DNA同源性和表达方法相结合，已分离出大鼠多巴胺（DA）转运体互补DNA。DA转运体是一种含有619个氨基酸的蛋白质，具有12个疏水的假定跨膜结构域，与去甲肾上腺素和γ-氨基丁酸转运体具有同源性。所表达的互补DNA赋予非洲爪蟾卵母细胞和COS细胞转运[3H]DA的能力。可卡因类似物[3H]CFT（[3H]2β-甲氧羰基-3β-（4-氟苯基）托烷）与转染的COS细胞膜结合产生的药理学特征与纹状体膜中的相似。
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