Hoffman B J, Mezey E, Brownstein M J
Laboratory of Cell Biology, National Institute of Mental Health, Bethesda, MD 20892.
Science. 1991 Oct 25;254(5031):579-80. doi: 10.1126/science.1948036.
A complementary DNA clone for a serotonin (5HT) transporter has been isolated from rat basophilic leukemia cells. The complementary DNA sequence predicts a 653-amino acid protein with 12 to 13 putative transmembrane domains. The 5HT transporter has significant homology to the gamma-aminobutyric acid, dopamine, and norepinephrine transporters. Uptake by CV-1 cells expressing the transporter complementary DNA resembles 5HT uptake by platelets and brain synaptosomes; it is sensitive to antidepressants, amphetamine derivatives, and cocaine.
从大鼠嗜碱性白血病细胞中分离出了一种血清素（5-羟色胺，5HT）转运体的互补DNA克隆。该互补DNA序列预测出一种含有12至13个推定跨膜结构域的653个氨基酸的蛋白质。5HT转运体与γ-氨基丁酸、多巴胺和去甲肾上腺素转运体具有显著的同源性。表达该转运体互补DNA的CV-1细胞的摄取类似于血小板和脑突触体对5HT的摄取；它对抗抑郁药、苯丙胺衍生物和可卡因敏感。
